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于荣荣1, 张育平1, 马恩波2, 张建珍2*.中华稻蝗Knickkopf(Knk)基因分子特性和生物学功能[J].植物保护,2020,46(4):74-80.
中华稻蝗Knickkopf(Knk)基因分子特性和生物学功能
Molecular characteristics and biological function of Knickkopf (Knk) gene in Oxya chinensis
投稿时间:2019-11-26  修订日期:2020-02-01
DOI:10.16688/j.zwbh.2019648
中文关键词:  中华稻蝗  Knickkopf  分子特性  生物学功能
英文关键词:Oxya chinensis  Knickkopf  molecular characteristics  biological function
基金项目:国家自然科学基金青年科学基金(31801749);山西省教育厅高等学校科技创新项目(2019L0796)
作者单位E-mail
于荣荣1, 张育平1, 马恩波2, 张建珍2* 1. 太原师范学院, 晋中 030619
2. 山西大学, 太原 030006 
zjz@sxu.edu.cn 
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中文摘要:
      基于中华稻蝗转录组数据库,采用生物信息学方法搜索获得1条OcKnk基因全长cDNA序列,采用qRT-PCR检测其组织部位表达特性和其在表皮发育过程中表达情况,明确其分子特性,采用RNAi技术结合表型观察,研究其对中华稻蝗蜕皮和生长发育的影响,以明确其生物学功能。组织部位表达结果显示其在中华稻蝗体壁、前肠和脂肪体表达最高,发育表达结果显示其在表皮不同发育日龄均有表达,且在蜕皮前期和后期表达显著高于其他日龄。生物学功能研究表明,注射dsRNA后,多数虫体难以成功蜕去旧表皮,导致死亡,少部分可蜕至下一龄期,但活动力较低,行动缓慢,最终死亡。研究结果表明OcKnk参与昆虫生长发育和蜕皮过程,可作为重要靶标基因,为下一步害虫防治提供理论基础。
英文摘要:
      The full-length cDNA of OcKnk gene was obtained based on the transcriptome database of Oxya chinensis. To determine the molecular characteristics of OcKnk, the expressions of OcKnk in different tissues and during different developmental stage (day) of cuticle were detected by qRT-PCR. The biological functions of OcKnk affecting molting and development were investigated by RNAi technology combined with phenotype observation. Tissue expression results indicated that there was a highest expression in the integument, foregut and fat body; developmental expression pattern showed that it was expressed in the cuticle during different developmental days of age, with a significantly higher expression during proecdysis and post-ecdysis. The biological function assay showed that most locusts were unable to escape from the old cuticle successfully, finally leading to death and failing to reach the next instar. During the process, they moved slowly with low activity and eventually went to death. The results indicated that OcKnk may be involved in insect growth and molting, which can be used as an important target gene and provide a theoretical basis for further pest control.
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