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孙少双, 王冬雪, 杨秀玲*, 周雪平.胜红蓟上一种与双生病毒伴随的重组β卫星分子的鉴定[J].植物保护,2020,46(4):19-24.
胜红蓟上一种与双生病毒伴随的重组β卫星分子的鉴定
Identification of a recombinant geminivirus-associated betasatellite from Ageratum conyzoides
投稿时间:2019-05-19  修订日期:2019-05-29
DOI:10.16688/j.zwbh.2019253
中文关键词:  胜红蓟  双生病毒  β卫星  重组
英文关键词:Ageratum conyzoides  geminivirus  betasatellite  recombination
基金项目:国家重点研发计划(2016YFD0300701);中央级公益性科研院所基本科研业务费专项(Y2017JC04)
作者单位E-mail
孙少双, 王冬雪, 杨秀玲*, 周雪平 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 xlyang@ippcaas.cn 
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中文摘要:
      双生病毒是一类在全球范围内危害严重的单链环状DNA病毒。本研究从2017年采自云南的胜红蓟样品中(YN2017)获得了一条菜豆金色花叶病毒属病毒和一条β卫星的全基因组序列。该双生病毒的全长序列与烟草曲茎病毒的相似性最高,为99.60%,确定为烟草曲茎病毒的分离物。YN2017 β卫星与中国胜红蓟黄脉β卫星的同源性最高,为90.8%。进一步分析发现,该β卫星的卫星保守区域(SCR)至富含腺嘌呤区(A-rich区)之间约1 kb的序列与中国胜红蓟黄脉β卫星相应序列的相似性高达97.2%;其包含的A-rich区上游与SCR之间约300 bp的序列与中国胜红蓟黄脉β卫星相应序列的相似性仅为70.2%,而与烟草曲茎β卫星相应序列的相似性最高,为97.3%。重组分析发现,所分离的β卫星是由中国胜红蓟黄脉β卫星和烟草曲茎β卫星重组产生。这是首次在中国发现由不同的β卫星重组产生的β卫星分子。
英文摘要:
      Geminiviruses are a group of single-stranded DNA viruses that cause severe losses to many crops worldwide. In this study, we determined the complete nucleotide sequences of a begomovirus and a betasatellite from an Ageratum conyzoides plant (YN2017) showing yellow vein and leaf curling symptoms in Yunnan. The begomovirus shared 99.60% sequence identity with Tobacco curly shoot virus (TbCSV) and thus was identified as an isolate of TbCSV. The YN2017 betasatellite shared the highest sequence identity (90.8%) with Ageratum yellow vein China betasatellite (AYVCNB). Further analysis of the betasatellite sequences showed that the sequences from the satellite conserved region (SCR) to the A-rich region shared 97.2%identity with the homologous sequences of AYVCNB, while the sequences between the SCR and the upstream A-rich region only shared 70.2%identity with that of AYVCNB. Instead, these sequences were more closely related (97.3%sequence identity) to the coordinated sequences of TbCSB. Recombination detection analysis showed that the betasatellite might result from the recombination of AYVCNB and TbCSB. This is the first report of a recombinant betasatellite that has arisen from betasatellites recombination in China.
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