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赵玉丹#, 朱晓颖#, 徐国娟, 王旭丽*.乙烯信号传导途径因子OsEIL 6调控水稻抗稻瘟病反应[J].植物保护,2020,46(4):12-18.
乙烯信号传导途径因子OsEIL 6调控水稻抗稻瘟病反应
OsEIL6 is involved in regulating rice resistance to Magnaporthe oryzae
投稿时间:2020-03-26  修订日期:2020-05-06
DOI:10.16688/j.zwbh.2020153
中文关键词:  水稻  稻瘟病  抗病性  乙烯  OsEIL6
英文关键词:Oryza sativa  rice blast  disease resistance  ethylene  OsEIL6
基金项目:国家自然科学基金(31671984,31471737); 转基因生物新品种培育科技重大专项(2012ZX08009001)
作者单位E-mail
赵玉丹#, 朱晓颖#, 徐国娟, 王旭丽* 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京 100193 wangxuli@caas.cn 
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中文摘要:
      稻瘟病(rice blast)是水稻生产上最严重的病害之一。抗病相关基因的挖掘对稻瘟病的防治具有重要意义。研究表明植物EIN3/EIL家族基因在抗病过程中发挥着重要作用。本研究采用RNAi技术探究OsEIL6参与的水稻抗稻瘟病反应。稻瘟菌侵染时基因表达谱检测结果表明,OsEIL6在水稻和稻瘟菌非亲和组合中受到诱导表达。稻瘟菌接种结果显示,水稻OsEIL6沉默株系和野生型植株‘TG394’相比抗性下降;实时荧光定量RT-PCR结果分析表明,OsEIL6的表达量下降导致乙烯合成途径中OsACO1和乙烯信号传导途径的OsERF063和OsERF073的转录水平下降。亚细胞定位研究发现该基因定位于水稻细胞质。OsEIL6沉默株系中ROS合成途径标记基因OsrbohA和OsrbohB的表达量均明显下调,表明该基因可能通过影响ROS的合成调控水稻抗稻瘟病反应。本研究结果将有助于进一步揭示OsEIL6参与的乙烯信号传导途径介导的水稻抗稻瘟病反应机制。
英文摘要:
      Rice blast is one of the most destructive rice diseases. Understanding the function of rice immune responsive genes to the blast fungus Magnaporthe oryzae is essential for effective disease management. Previous research revealed that ethylene-insensitive 3 (EIN3) /ethylene-insensitive 3-like (EIL) gene plays essential roles in plant defense responses. In this study, we investigated the role of OsEIL6 in rice resistance to M. oryzae. Expression of OsEIL6 was significantly induced in rice after infection with an incompatible blast isolate C9240. Silencing of OsEIL6 in transgenic rice enhanced plant susceptibility to M.oryzae. Quantitative RT-PCR assays showed that the transcript levels of OsACO1 in ethylene biosynthesis, and two key downstream genes, OsERF063 and OsERF073, in ethylene signaling pathways, ware down-regulated in the OsEIL6-RNAi plants. Subcellular localization analyses showed that the OsEIL6 protein was localized in the cytoplasm of rice cells. In addition, the expression levels of OsrbohA and OsrbohB, two key genes for ROS biosynthesis, were down-regulated in the OsEIL6-RNAi plants, suggesting that OsEIL6 may mediate rice resistance by affecting the ROS production. Our findings will contribute to further understanding of the mechanism of OsEIL6-mediated resistance to M. oryzae.
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