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田守波, 朱为民, 朱龙英, 杨学东, 张迎迎, 张 辉*, 刘 娜*.番茄棒孢叶斑病病原鉴定及生物学特性研究[J].植物保护,2020,46(3):157-162.
番茄棒孢叶斑病病原鉴定及生物学特性研究
Identification of the pathogen from Corynespora leaf spot of tomato and research on its biological characteristics
投稿时间:2019-02-28  修订日期:2019-04-16
DOI:DOI: 10.16688/j.zwbh.2019091
中文关键词:  番茄棒孢叶斑病  多主棒孢  病原鉴定  生物学特性
英文关键词:Corynespora leaf spot of tomato  Corynespora cassiicola  pathogen identification  biological characteristics
基金项目:上海市农委青年人才成长计划项目(沪农青字(2018)第1-15号);上海市科技兴农项目(沪农科推字(2019)第3-1号)
作者单位E-mail
田守波, 朱为民, 朱龙英, 杨学东, 张迎迎, 张 辉*, 刘 娜* 上海市农业科学院设施园艺研究所, 上海市设施园艺技术重点实验室, 上海 201403 E-mail:张辉zhanghui@saas.sh.cn;刘娜yyliuna@163.com 
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中文摘要:
      本研究采用PSA培养基对上海地区的番茄棒孢叶斑病的病原菌进行分离、培养及致病性测定,通过形态学和分子生物学方法鉴定病原菌,并对该病菌的生物学特性进行研究。结果表明:番茄棒孢叶斑病病原菌菌落呈灰褐色,菌丝质地致密、绒毛状;分生孢子单生或串生,圆柱形或倒棍棒形、顶端钝圆,基部平截,呈半透明至浅褐色,假隔膜4~10个,直或稍弯曲,基脐加厚,深褐色。结合病原菌rDNA ITS序列测定、比对后,确定该病原为多主棒孢Corynespora cassiicola。该病菌的菌丝生长最适温度为25~30℃,最适pH范围为4~8,产孢的最适温度约为25℃,最适pH范围为5~9,麦芽糖和乳糖为碳源的培养基适宜菌丝生长,可溶性淀粉和乳糖溶液中孢子萌发率最高,光暗交替条件适宜菌丝生长,孢子在水滴中极易萌发。这是该病在上海地区的首次报道。
英文摘要:
      In this study, PSA was used to isolate culture and test the pathogen of Corynespora leaf spot of tomato in Shanghai. Morphological and molecular biological methods were used to identify the pathogen, and its biological characteristics were studied. The results showed that the colony of the pathogen was grayish brown with dense and villous hyphae; conidia were solitary or connate, cylindrical or inverted rod-shaped, obtuse-rounded at the top, flat at the base, translucent to light brown; pseudomembranes were 4-10, straight or slightly curved, and basal umbilicus was thicker and dark brown. Combined with rDNA ITS sequence analysis and comparison, the pathogen was identified as Corynespora cassiicola. The optimum temperature for mycelial growth and sporulation of the pathogen was 25-30℃ and 25℃, respectively. The optimum pH value was 4-8 and 5-9 respectively. The medium with maltose and lactose as carbon source was suitable for mycelial growth. The spore germination rate in soluble starch and lactose solution was the highest. The alternating light and dark conditions were suitable for mycelial growth and spores germinate easily in water droplets. This was the first report of the disease in Shanghai.
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