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马俊丰1,2, 李小宇2, 张春雨2, 周雪平3*, 王永志2*.甘薯G病毒吉林分离物的全基因组序列测定与分析[J].植物保护,2020,46(3):57-61.
甘薯G病毒吉林分离物的全基因组序列测定与分析
Complete genome sequencing and analysis of Sweet potato virus G isolate from Jilin, China
投稿时间:2019-04-02  修订日期:2019-06-01
DOI:DOI: 10.16688/j.zwbh.2019163
中文关键词:  甘薯G病毒(SPVG)  全基因组  比对分析  系统发育
英文关键词:Sweet potato virus G (SPVG)  complete genome  comparative analysis  phylogeny
基金项目:国家重点研发计划(2017YFD0201604);吉林省农业科技创新工程(CXGC2017JQ021);吉林省农业产业技术体系马铃薯(甘薯)综合栽培技术示范与推广(2018)
作者单位E-mail
马俊丰1,2, 李小宇2, 张春雨2, 周雪平3*, 王永志2* 1. 吉林农业大学, 长春 130118
2. 吉林省农业科学院, 公主岭 136100
3. 中国农业科学院植物保护研究所, 北京 100193 
E-mail:周雪平zzhou@zju.edu.cn; 王永志yzwang@126.com 
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中文摘要:
      利用RT-PCR结合RACE方法,从感染SPVG的甘薯叶片中获得甘薯G病毒吉林分离物Jilin-gzl的全基因组序列。测序获得的Jilin-gzl分离物(GenBank No.Mk392509)基因组为10 797 nt。该病毒基因组含有一个10 464 nt的开放阅读框,编码由3 488个氨基酸构成的多聚蛋白。在P1和P3基因中也发现了由移码翻译产生的PISPO蛋白和PIPO蛋白。比对分析显示,在开放阅读框水平上Jilin-gzl与6个不同分离物核苷酸序列一致性为79%~99%,氨基酸一致性为92%~99%,其中与SC11、IS103、HG167和Jesus_Maria分离物一致性最高,与WT325和AI一致性最低。系统发育分析显示,Jilin-gzl与HG167和WCFR11系统发育关系最近,与中国台湾地区分离物WT325系统发育关系较远。这是中国大陆地区关于SPVG分离物全基因序列的首次报道,同时也是首次在中国东北地区发现SPVG。该研究探明了Jilin-gzl分离物的基因结构,系统发育关系,丰富了SPVG基因组序列信息,为后续开展SPVG种群的遗传进化及功能研究奠定了基础。
英文摘要:
      The complete genome of Jilin-gzl was determined by using rapid-amplification of cDNA ends (RACE) and RT-PCR. The complete sequence of Jilin-gzl (GenBank No.Mk392509) had 10 797 nucleotides, excluding the 3′-terminal poly (A) tail. Its genome contained an open reading frame of 10 464 nucleotides and encoded a polyprotein of 3 488 amino acids. Two additional proteins, termed ‘PISPO’ and ‘PIPO’, were also translated by frame shifting within the P1 and P3 cistron. The results of sequence identity analysis of Jilin-gzl isolate with the 6 reference SPVG isolates showed that Jilin-gzl isolate shared a 79%-99% nucleotide identity and 92%-99% amino acid identity with other SPVG isolates at the ORF sequence level. Jilin-gzl isolate shared a highest sequence identity with SC11, IS103, HG167, Jesus_Maria isolates, and a lowest identity with WT325 and AI isolates. Phylogenetic analysis indicated that Jilin-gzl shared high sequence homology with HG167 and WCFR11 isolates, and shared low sequence homology with WT325 isolate from Taiwan, China. This work was the first report about the complete sequence of SPVG in Mainland China and it was also the first time that SPVG has been found in Northeast China. This research elucidated the genetic structure and phylogenetic relationship of Jilin-gzl isolate, and enriched the information of SPVG genome sequence. These will provide useful information for further study of the phylogenesis and function of this pathogen.
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