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赵丹丹, 陈景超, 黄兆峰, 姜翠兰, 黄红娟*,张朝贤, 李香菊, 魏守辉*.刺萼龙葵种子中适宜内参基因的筛选[J].植物保护,2020,46(3):40-46.
刺萼龙葵种子中适宜内参基因的筛选
Selection of suitable reference genes in Solanum rostratum seeds
  修订日期:2019-04-02
DOI:DOI: 10.16688/j.zwbh.2019105
中文关键词:  刺萼龙葵  内参基因  种子  赤霉素  脱落酸
英文关键词:Solanum rostratum  reference gene  seed  gibberellin  abscisic acid
基金项目:国家自然科学基金(31572022);国家重点研发计划(2018YFD0200602);北京市自然科学基金(6182033)
作者单位E-mail
赵丹丹, 陈景超, 黄兆峰, 姜翠兰, 黄红娟*,张朝贤, 李香菊, 魏守辉* 中国农业科学院植物保护研究所, 中国农业科学院杂草鼠害生物学与治理重点开放实验室, 北京 100193 E-mail:黄红娟hjhuang@ippcaas.cn;魏守辉shwei@ippcaas.cn 
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中文摘要:
      入侵杂草刺萼龙葵Solanum rostratum Dunal传播扩散的主要载体是种子,研究其种子休眠萌发基因的激素调控对于其防除具有重要意义,而选择合适的内参基因可以提高相关基因表达分析的准确性。本研究以赤霉素、脱落酸和水处理的刺萼龙葵种子为材料,利用geNorm、NormFinder、BestKeeper和RefFinder 4种软件对15个候选内参基因进行表达稳定性评价,并通过检测ABI5(abscisic acid-insensitive 5)的表达验证所筛选的内参基因的适用性。结果表明,对于赤霉素、脱落酸和水处理过的种子,最稳定的内参基因分别为eIF(eukaryotic initiation factor)、SAND(SAND protein family)和ACT(β-actin);对所有种子样本而言,PP2Acs(a catalytic subunit of protein phosphatase 2A)是最稳定的内参基因。研究结果将为刺萼龙葵种子休眠萌发的遗传调控研究提供重要参考。
英文摘要:
      The main transmission way of the invasive weed buffalobur (Solanum rostratum) is seed dispersal. It is important to understand the hormonal regulation of seed dormancy or germination related genes in buffalobur. Selection of appropriate reference genes can improve the accuracy of expression analysis of these genes. Here, 15 candidate reference genes were selected to evaluate their expression stability in hormone-treated and water-treated seeds of buffalobur using geNorm, NormFinder, BestKeeper and RefFinder statistical algorithms. Validation of recommended reference genes were achieved by assessing the relative expression levels of abscisic acid-insensitive 5 (ABI5). The results showed that the most stable reference genes for gibberellin, abscisic acid and water-treated seeds were eukaryotic initiation factor (eIF), SAND protein family (SAND) and β-actin (ACT), respectively. For total seeds, PP2Acs, the gene encoding a catalytic subunit of protein phosphatase 2A was the most stable reference gene. This work would provide references for further research on the genetic regulation of seed dormancy and germination in this invasive plant.
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