| 黄婉琴, 李 勤, 覃章辉, 王明红*.茭白胡麻叶斑病病原菌分离与鉴定[J].植物保护,2020,46(2):129-134. |
| 茭白胡麻叶斑病病原菌分离与鉴定 |
| Isolation and identification of the pathogen causing leaf spot of Zizania latifolia |
| 投稿时间：2019-02-02 修订日期：2019-04-07 |
| DOI：10.16688/j.zwbh.2019060 |
| 中文关键词: 茭白胡麻叶斑病 病原菌鉴定 稻平脐蠕孢 |
| 英文关键词:Zizania latifolia leaf spot disease pathogen identification Bipolaris oryzae |
| 基金项目:湖北省技术创新专项重大项目(2017ABA152);中央引导地方科技发展专项(［2016］150) |
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| 中文摘要: |
| 茭白胡麻叶斑病发生普遍且严重,已成为茭白的常见病害。为了明确病原菌,先后从茭白主产区采集胡麻叶斑病病叶,各产区分离纯化5~10株病原菌。通过对菌株DNA的内转录间隔区ITS基因、3-磷酸甘油醛脱氢酶GPDH基因、延伸因子EF-1α基因的序列进行分析。结合形态学观察、致病力测定等方法对茭白胡麻叶斑病病菌进行鉴定。结果表明,茭白胡麻叶斑病病原菌为稻平脐蠕孢Bipolaris oryzae,与水稻胡麻叶斑病病原菌一致。本文的研究成果可为茭白胡麻叶斑病的防治提供一种新的思路。 |
| 英文摘要: |
| Zizania latifolia leaf spot disease is a common and serious disease. In order to identify the pathogen, we collected infected leaves from different main production areas of Z. latifolia and selected 5 to 10 strains in each area. Identification experiments were performed based on DNA internal transcribed spacer ITS, glyceraldehyde-3-phosphate dehydrogenase (GDPH) and elongation factor EF-1α gene sequences and morphology. The results showed that the pathogen of leaf spot of Z. latifolia was Bipolaris oryzae, which was the same as the pathogen of rice flax leaf spot. This study provides a new idea for the control of leaf spot of Z. latifolia. |
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