| 王天旗1, 史晓斌2, 郑立敏2, 刘 勇2, 张德咏2, 谭新球2, 周序国1*.番茄褪绿病毒侵染黄瓜的首次报道[J].植物保护,2020,46(2):91-95. |
| 番茄褪绿病毒侵染黄瓜的首次报道 |
| Cucumber (Cucumis sativus) is a new host for Tomato chlorosis virus |
| 投稿时间：2019-03-04 修订日期：2019-04-07 |
| DOI：10.16688/j.zwbh.2019100 |
| 中文关键词: 番茄褪绿病毒 黄瓜 烟粉虱 RT-PCR |
| 英文关键词:Tomato chlorosis virus Cucumis sativus Bemisia tabaci RT-PCR |
| 基金项目:国家自然科学基金（31872932，31672003）; 湖南省科技计划（2016RS2019）; 国家大宗蔬菜产业技术体系(CARS-25-B-05) |
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| 中文摘要: |
| 番茄褪绿病毒Tomato chlorosis virus(ToCV)是严重危害世界经济作物的一种病毒,寄主范围广泛。田间调查发现黄瓜Cucumis sativus表现出叶片黄化、脉间褪绿的疑似番茄褪绿病毒感病症状,同时叶片背面聚集了大量烟粉虱。采用RT-PCR方法对样品叶片和烟粉虱进行检测,ToCV感染率为65%,且发病叶片上烟粉虱携带ToCV。为进一步确定黄瓜是否为番茄褪绿病毒的新寄主,室内利用农杆菌侵染性克隆接种健康黄瓜,结果显示:接种30 d的黄瓜新生叶片出现褪绿症状。采用ToCV HSP70基因的引物对田间黄瓜叶片、烟粉虱和室内黄瓜新生叶片进行RT-PCR,扩增出约450 bp的条带,在NCBI上BLAST显示与KC887999.1的同源性最高,为99%。这些数据表明黄瓜是番茄褪绿病毒的寄主。这是ToCV感染黄瓜的首次报道。 |
| 英文摘要: |
| Tomato chlorosis virus (ToCV) causes severe damage to crops worldwide, and it infects a wide range of plant hosts. In a field investigation, we found leaf etiolation, interveinal chlorosis of suspected symptoms of tomato chlorosis virus disease in cucumber (Cucumis sativus); meanwhile a large number of Bemisia tabaci gathered on the back of cucumber leaves. The leaves and B.tabaci were tested by RT-PCR. The results showed that the ToCV infection rate was 65%, and B.tabaci on the infected leaves carried ToCV. In order to further determine whether cucumber is the new host of tomato chlorosis virus, healthy cucumbers were inoculated with agrobacterium-infected clones indoors. The results showed that chlorosis appeared in new leaves of cucumbers 30 days after inoculation. The primers of ToCV HSP70 gene were used to perform RT-PCR on cucumber leaves, whiteflies and new leaves of indoor cucumber, and a band of 450 bp was amplified. BLAST on NCBI showed the highest homology (99%) with KC887999.1. These data indicate that cucumber is the host of tomato chlorosis virus. This is the first report of ToCV infection in cucumbers. |
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