| 黄建华 1, 孙 强 2, 杨迎青 1, 兰 波 1, 李湘民 1*.少毛钝绥螨的生物学特性及rDNA ITS序列分析[J].植物保护,2020,46(1):208-211. |
| 少毛钝绥螨的生物学特性及rDNA ITS序列分析 |
| Biological characteristics and rDNA ITS sequence analysis of Proprioseiopsis asetus (Acari: Phytoseiidae) |
| 投稿时间：2018-12-27 修订日期：2019-01-11 |
| DOI：DOI: 10.16688/j.zwbh.2018533 |
| 中文关键词: 少毛钝绥螨 生物学特性 rDNA ITS 序列分析 系统发育 |
| 英文关键词:Proprioseiopsis asetus biological characteristics rDNA ITS sequence analysis phylogenetic relationship |
| 基金项目:江西省科技支撑计划（20122BBF60103）；公益性行业（农业）科研专项（200903032）；国家自然科学基金（31460456）；江西省杰出青年人才计划（20171BCB23081） |
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| 中文摘要: |
| 少毛钝绥螨Proprioseiopsis asetus是在国内有分布的一种捕食螨,其研究尚不深入。为明确其生物学特性,在25℃室内条件下研究了少毛钝绥螨对芦笋蓟马1龄若虫、柑橘全爪螨低龄若螨、椭圆食粉螨1龄若螨和丝瓜花粉的食性。结果表明:少毛钝绥螨可以取食以上4种食物,其中对粉螨和芦笋蓟马的捕食量较大,对柑橘全爪螨捕食量较小。少毛钝绥螨及其近缘种的rDNA ITS序列在61～85、116～159、177～198、224～254、277～317、531～549、438～445 bp和580～594 bp等ITS区段存在差异性碱基。少毛钝绥螨及其近缘种分为两个组群。Proprioseiopsis、Neoseiulus、Amblyseius聚为一个组群(Group Ⅰ),而Euseius单独聚为一个组群(Group Ⅱ)。少毛钝绥螨与N. womersleyi和N. agrestis亲缘关系较近。 |
| 英文摘要: |
| Proprioseiopsis asetus (Chant) (Acari: Phytoseiidae) is a widespread predatory mite in China, and it is poorly investigated. We studied the feeding habits of P. asetus reared on the first instar nymphs of Thrips tabaci Lindeman, Aleuroglyphus ovatus Troupeau, Panonychus citri McGregor and Luffa cylindrical Roem pollen. The results showed that P. asetus could feed on the above four foods; A. ovatus and T. tabaci were the most consumed preys, and P. citri was the least consumed . The molecular identification results revealed that its ITS sequence shared an identity of 100% with that of P. asetus and it was therefore identified as P. asetus. There existed differential bases at the 61－85 bp, 116－159 bp, 177－198 bp, 224－254 bp, 277－317 bp, 531－549 bp, 438－445 bp and 580－594 bp fragments of ITS sequences between P. asetus and its related predatory mites. P. asetus and its related predatory mites clustered as 2 groups, in which Proprioseiopsis , Neoseiulus and Amblyseius clustered as a group (Group Ⅰ), while Euseius clustered as another group (Group Ⅱ). P. asetus was phylogenetically closer to N. womersleyi and N. agrestis . |
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