张照茹 1, 魏松红 1*, 杨晓贺 1, 2, 王海宁 1, 许 月 1.中国东北地区水稻纹枯病病原菌种类及融合群的分子鉴定[J].植物保护,2019,45(6):283-287. |
中国东北地区水稻纹枯病病原菌种类及融合群的分子鉴定 |
Molecular identification of pathogens and anastomosis groups of rice sheath blight in Northeast China |
投稿时间:2018-10-09 修订日期:2018-11-22 |
DOI:10.16688/j.zwbh.2018435 |
中文关键词: 水稻纹枯病 茄丝核菌 水稻丝核菌 融合群 |
英文关键词:rice sheath blight Rhizoctonia solani Rhizoctonia oryzae-sativae anastomosis group |
基金项目:辽宁省“百千万人才工程”资助项目; 现代农业产业技术体系建设专项(CARS-01) |
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中文摘要: |
为明确中国东北地区水稻纹枯病病原菌种类及融合群的归属情况, 2015-2017年从黑龙江省、吉林省和辽宁省的17个水稻主产区采集水稻纹枯病标样, 分离获得水稻纹枯病菌214株, 运用水稻纹枯病菌的不同病原菌及融合群的特异性引物对214株水稻纹枯病菌进行病原菌种类和融合群鉴定, 并利用rDNA内转录间隔区(ITS)序列, 对供试水稻丝核菌的融合群归属进行了分析。结果表明:供试214株水稻纹枯病菌分属于茄丝核菌Rhizoctonia solani和水稻丝核菌Rhizoctonia oryzae-sativae, 菌株数分别为198株和16株, 占比分别为92.52%和7.48%。茄丝核菌菌株分属于2个融合群, 分别为AG1-IA和AG4, 菌株数分别为191株和7株, 占比分别为96.46%和3.54%。水稻丝核菌菌株均属于AG-Bb融合群, 菌株数为16株。不同年份水稻纹枯病的病原菌种类及融合群出现的频率和地域分布无明显变化, 而不同地域间水稻纹枯病病原菌的种类及融合群具有明显的分化特征, AG1-IA融合群在中国东北三省各个水稻产区均有分布且均为优势融合群, AG4融合群在辽宁省盘锦市出现频率最高, 水稻丝核菌AG-Bb融合群在吉林省吉林市、通化市和梅河口市出现频率最高。 |
英文摘要: |
In order to elucidate the pathogens and anastomosis group of rice sheath blight in Northeast China from 2015 to 2017, 214 strains of rice sheath blight were isolated from 17 main rice producing areas in Heilongjiang, Jilin and Liaoning provinces. The specific primers of different pathogens and anastomosis groups of rice sheath blight pathogen were used to identify the pathogens and anastomosis group of 214 isolates, and sequence of the internal transcription spacer of rDNA (ITS) was used to analyze the belonging of the anastomosis group of Rhizoctonia oryzae-sativae. The results showed that 214 isolates belonged to two pathogens. For Rhizoctonia solani and R.oryzae-sativae, the number of strains was 198 and 16, accounting for 92.52% and 7.48%, respectively. R.solani strains belong to two anastomosis groups, the number of AG1-IA and AG4 strains was 191 and 7 accounting for 96.46% and 3.54%, respectively. Phylogenetic analysis of ITS sequence showed that R.oryzae-sativae belonged to AG-Bb. In different years, the occurrence frequency and regional distribution of different populations and anastomosis groups of rice sheath blight pathogen had no significant change, but different populations and anastomosis groups of rice sheath blight pathogen in different regions had obvious differentiation characteristics. AG1-IA anastomosis group distributed in all rice producing areas, and it was the major anastomosis group with the highest frequency in Panjin, Liaoning province. The AG-Bb anastomosis group of R.oryzae-sativae appeared most frequently in Jilin city, Tonghua city and Meihekou city, Jilin province. |
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