| 柳吉芹 1, 王立杉 1, 2, 吴 曦 1, 钱云开 1, 高 飞 1, 王海洋 1*.加拿大进口大麦中小麦线条花叶病毒检疫鉴定[J].植物保护,2019,45(6):265-268. |
| 加拿大进口大麦中小麦线条花叶病毒检疫鉴定 |
| Identification of Wheat streak mottle virus on imported barley from Canada |
| 投稿时间：2018-09-20 修订日期：2018-11-01 |
| DOI：10.16688/j.zwbh.2018409 |
| 中文关键词: 小麦线条花叶病毒 大麦 PCR ELISA 序列比对分析 |
| 英文关键词:Wheat streak mosaic virus barley PCR ELISA sequence alignment analysis |
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| 中文摘要: |
| 小麦线条花叶病毒(WSMV)是我国进境植物检疫性有害生物。2018年5月秦皇岛海关利用反转录PCR(RT-PCR)和双抗体夹心酶联免疫吸附法(DAS-ELISA), 从加拿大进口的大麦中检出WSMV。同时利用实时荧光定量PCR (Real time RT-qPCR)和序列比对分析方法进行了验证。这是我国首次在进境加拿大大麦中截获WSMV。 |
| 英文摘要: |
| Wheat streak mosaic virus (WSMV) was one of the most important quarantine pests in China, which had caused significant economic losses in USA. WSMV was detected in the samples of imported barley from Canada using reverse transcription PCR (RT-PCR) and double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) methods by Qinhuangdao custom in May 2018, which was further confirmed by real time RT-qPCR and sequence alignment analysis. This was the first report on interception of WSMV on imported barley from Canada. |
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