| 王诗玮, 纪 琪, 朱天辉 *.紫外线诱变莱氏绿僵菌对敌敌畏的耐药性[J].植物保护,2019,45(6):185-191. |
| 紫外线诱变莱氏绿僵菌对敌敌畏的耐药性 |
| Resistance of Metarhizium rileyi UV mutation to dichlorvos |
| 投稿时间：2018-11-03 修订日期：2019-02-25 |
| DOI：10.16688/j.zwbh.2018465 |
| 中文关键词: 紫外线诱变 莱氏绿僵菌 敌敌畏 药剂理化诱变 耐药性 |
| 英文关键词:UV mutagenesis Metarhizium rileyi dichlorvos drug mutagenesis drug resistance |
| 基金项目:中国博士后基金面上项目（2016M602705） |
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| 中文摘要: |
| 为筛选出对常用有机磷杀虫剂敌敌畏具有较强耐药性的莱氏绿僵菌Metarhizium rileyi突变菌株, 本文通过紫外线照射诱变莱氏绿僵菌, 并利用敌敌畏对紫外突变菌株进行4次药剂理化诱变, 并对突变株进行了抗性水平、遗传稳定性测定, 得出敌敌畏对莱氏绿僵菌孢子的致死浓度为1 291 mg/L, 适合莱氏绿僵菌紫外线诱变的最佳照射时间为2 min和2.5 min, 得到6株能耐1 291 mg/L敌敌畏的突变菌株, 其中突变株Nr-UVY6的抗性水平是出发菌株的2.57倍, 抗药性遗传稳定性也最佳。 |
| 英文摘要: |
| In order to screen out the mutant strain of Metarhizium rileyi which is highly resistant to dichlorvos, a common organophosphate insecticide. The M. rileyi strain mutagenized by ultraviolet irradiation, and DDVP was used to domesticate the mutant strain for 4 times. The resistance level and genetic stability of the mutant were determined. The lethal concentration of dichlorvos against the spores of M.rileyi was 1 291 mg/L, and the optimal durations fort UV irradiation were 2 min and 2.5 min. Six mutant strains resistant to 1 291 mg/L DDVP were obtained. The resistance level of the mutant strain Nr-UVY6 was 2.57 times higher than the original strain, and the genetic stability of the drug resistance was also the best. |
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