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王诗玮, 纪 琪, 朱天辉 *.紫外线诱变莱氏绿僵菌对敌敌畏的耐药性[J].植物保护,2019,45(6):185-191.
紫外线诱变莱氏绿僵菌对敌敌畏的耐药性
Resistance of Metarhizium rileyi UV mutation to dichlorvos
投稿时间:2018-11-03  修订日期:2019-02-25
DOI:10.16688/j.zwbh.2018465
中文关键词:  紫外线诱变  莱氏绿僵菌  敌敌畏  药剂理化诱变  耐药性
英文关键词:UV mutagenesis  Metarhizium rileyi  dichlorvos  drug mutagenesis  drug resistance
基金项目:中国博士后基金面上项目(2016M602705)
作者单位E-mail
王诗玮, 纪 琪, 朱天辉 * 四川农业大学林学院, 温江 611130 zhuth1227@126.com 
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中文摘要:
      为筛选出对常用有机磷杀虫剂敌敌畏具有较强耐药性的莱氏绿僵菌Metarhizium rileyi突变菌株, 本文通过紫外线照射诱变莱氏绿僵菌, 并利用敌敌畏对紫外突变菌株进行4次药剂理化诱变, 并对突变株进行了抗性水平、遗传稳定性测定, 得出敌敌畏对莱氏绿僵菌孢子的致死浓度为1 291 mg/L, 适合莱氏绿僵菌紫外线诱变的最佳照射时间为2 min和2.5 min, 得到6株能耐1 291 mg/L敌敌畏的突变菌株, 其中突变株Nr-UVY6的抗性水平是出发菌株的2.57倍, 抗药性遗传稳定性也最佳。
英文摘要:
      In order to screen out the mutant strain of Metarhizium rileyi which is highly resistant to dichlorvos, a common organophosphate insecticide. The M. rileyi strain mutagenized by ultraviolet irradiation, and DDVP was used to domesticate the mutant strain for 4 times. The resistance level and genetic stability of the mutant were determined. The lethal concentration of dichlorvos against the spores of M.rileyi was 1 291 mg/L, and the optimal durations fort UV irradiation were 2 min and 2.5 min. Six mutant strains resistant to 1 291 mg/L DDVP were obtained. The resistance level of the mutant strain Nr-UVY6 was 2.57 times higher than the original strain, and the genetic stability of the drug resistance was also the best.
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