| 崔 佳 1, 赵丰舟 1, 刘 震 2, 曲建楠 1, 刘 铜 2*, 左豫虎 1*.玉米弯孢叶斑病菌多聚半乳糖醛酸酶Clpg1基因功能验证[J].植物保护,2019,45(6):163-169. |
| 玉米弯孢叶斑病菌多聚半乳糖醛酸酶Clpg1基因功能验证 |
| Functional verification of polygalacturonase Clpg 1 gene in Curvularia lunata causing maize Curvalaria leaf spot |
| 投稿时间：2018-11-23 修订日期：2019-01-06 |
| DOI：10.16688/j.zwbh.2018485 |
| 中文关键词: 玉米弯孢叶斑病菌 Clpg1 基因敲除 生物学特性 致病性 |
| 英文关键词:Curvularia lunata Clpg1 gene knockout biological characteristics pathogenicity |
| 基金项目:海南大学高层次人才科研启动基金（KYQD（ZK）1851） |
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| 中文摘要: |
| 为明确玉米弯孢叶斑病菌的多聚半乳糖醛酸酶基因Clpg1在其致病过程中的作用, 采用Double-joint PCR技术构建融合片段, 利用PEG介导的原生质体转化技术获得18株抗潮霉素突变体, 经检测得到1株Clpg1基因敲除突变体ΔClpg1。通过与野生型菌株比较, 发现ΔClpg1产孢量和孢子萌发率下降、多聚半乳糖醛酸酶活性降低, 致病力减弱。这些结果表明Clpg1基因影响多聚半乳糖醛酸酶活性, 调控了玉米弯孢叶斑病菌的致病性。 |
| 英文摘要: |
| In order to clarify the role of polygalacturonase Clpg1 gene in the pathogenicity of Curvularia lunata, the fusion fragment was constructed using double-joint PCR, and 18 hygromycin-resistant mutants were obtained by using PEG-mediated protoplast transformation technique. A knock-out mutant ΔClpg1 which contained no Clpg1 gene was found. Compared with the wild-type strain, the sporulation yield and germination rate of ΔClpg1 decreased; the polygalacturonase activity was reduced, and the pathogenicity was weakened. These results indicate that the Clpg1 gene affects the polygalacturonase activity and regulates the pathogenicity of Curvularia lunata. |
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