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周园园 1, 吴治然 1, 贡长怡 1, 韦朝领 2*, 张立新 1*.茶树链格孢叶斑病的病原鉴定[J].植物保护,2019,45(6):145-148.
茶树链格孢叶斑病的病原鉴定
Identification of Alternaria alternata causing leaf spot disease of Camellia sinensis
投稿时间:2018-10-28  修订日期:2019-02-14
DOI:10.16688/j.zwbh.2018458
中文关键词:  茶树叶斑病  链格孢  致病性  鉴定
英文关键词:leaf spot disease of tea plant  Alternaria alternata  pathogenicity  identification
基金项目:安徽农业大学茶树生物学与资源利用国家重点实验室开放基金(SKLTOF20170108); 安徽省科技重大专项(18030701211)
作者单位E-mail
周园园 1, 吴治然 1, 贡长怡 1, 韦朝领 2*, 张立新 1* 1.安徽农业大学植物保护学院, 合肥 230036
2. 安徽农业大学茶树生物学与资源利用国家重点实验室, 合肥 230036 
韦朝领weichl@ahau.edu.cn; 张立新lxzhang@ahau.edu.cn 
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中文摘要:
      茶树是我国重要的经济作物, 茶园叶病的流行会造成严重的经济损失。2017年10月到2018年1月从安徽、福建和湖北省茶区的9 个茶树品种上采集代表性茶叶斑病叶, 该病害的发病症状与由Colletotrichum spp.引起的茶炭疽病相似。采用组织分离法从发病叶片组织分离获得26 株菌落形态一致的真菌分离物, 显微镜观察结果显示, 各菌株分生孢子的产生方式和形态特征相似。为进一步明确菌株的分类地位, 选取2株来自安徽庐江和宣城地区的代表性菌株(EC-6和XBC1-3)进行多基因片段的PCR扩增和序列分析。结果表明, 代表性菌株EC-6和XBC1-3的ITS、gpd、tef-1a基因序列分别与交链格孢Alternaria alternata参考菌株CBS 107.27的序列(KP124300, KP124157, KP125075)相似性为100%、99%和100%, 结合菌株形态学观察以及柯赫氏法则验证, 证实交链格孢是引起该茶树叶斑病的致病菌。这是在安徽茶区首次发现由致病性链格孢引起茶树叶斑病。
英文摘要:
      The tea plant, Camellia sinensis (L.) O. Kuntze, is a crucial commercial crop in China. The foliar diseases often resulted in serious production loss during epidemic outbreak in tea plantations in China. From October 2017 to January 2018, representative diseased leaves showing similar symptoms with anthracnose disease were collected from tea plants of nine varieties in tea plantations in Anhui, Fujian and Hubei provinces. A total of 26 isolates were recovered consistently from symptomatic leaves by tissue isolation, and identified as Alternaria sp. by morphological features. The two strains (EC-6, XBC1-3) from Lujiang county and Xuancheng regions in Anhui province, respectively, were representatively selected for further identification by PCR amplification and sequence analysis. Using BLAST analysis of NCBI, the internal transcribed spacer (ITS) region, the gpd and tef-1a gene sequences of the two strains shared 100%, 99% and 100% sequence identity with those of the A.alternata strain CBS107.27 in GenBank (KP124300, KP124157, KP125075), respectively. Based on the morphological characters, sequences analysis as well as pathogenicity tests, the causal pathogen was identified as A. alternata. This is the first report of A. alternata causing leaf spot disease of tea plants in Anhui province.
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