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张 坤, 陈 雯, 甘海峰, 罗云建, 陈夕军, 贺 振 *.江苏省扬州市水仙病毒检测鉴定与序列分析[J].植物保护,2019,45(6):106-113.
江苏省扬州市水仙病毒检测鉴定与序列分析
Detection, identification and sequence analysis of narcissus viruses in Yangzhou city, Jiangsu province
投稿时间:2018-10-21  修订日期:2019-01-02
DOI:10.16688/j.zwbh.2018448
中文关键词:  水仙病毒  检测  序列分析
英文关键词:narcissus viruses  detection  sequence analysis
基金项目:国家自然科学基金(31601604, 31500388); 江苏省重点研发计划(现代农业)项目(BE2018359)
作者单位E-mail
张 坤, 陈 雯, 甘海峰, 罗云建, 陈夕军, 贺 振 * 扬州大学园艺与植物保护学院, 扬州 225009 hezhen@yzu.edu.cn 
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中文摘要:
      水仙属于石蒜科水仙属, 是重要的观赏花卉之一。2016年-2017年, 在江苏省扬州市采集的水仙上发现典型的花叶、条斑等病毒病症状。利用已报道的水仙病毒通用引物对疑似病样进行RT-PCR检测, 结果表明, 扬州采集到的3份病样上均能扩增到与预期大小相同的DNA片段。经克隆测序和序列分析发现, 所得序列包含3种不同的类型, 分别与水仙普通潜隐病毒 Narcissus common latent virus (NCLV)、水仙退化病毒 Narcissus degeneration virus (NDV)和水仙黄条病毒Narcissus yellow stripe virus (NYSV)有93%、98%和99%的相似性, 表明江苏水仙受到了NCLV、NDV、NYSV 3种病毒侵染。这是江苏水仙上第一次发现NCLV、NDV、NYSV。在系统树中, NCLV可分为两个组, 本研究所得NCLV-YZ与上海分离物位于同一组中; NDV也可分为两个组, 本研究所得NDV与其他中国分离物均位于第二组; 而NYSV具有很高的多样性, 可分为4个组, 本研究所得NYSV与其他部分中国分离物和日本分离物位于第一组。NCLV和NDV分组具有清晰的地理差异性, 而NYSV受到地理隔离的影响不明显。
英文摘要:
      Narcissus is an important ornamental flower, which belongs to the family Amaryllidaceae. In 2016-2017, typical viral infection symptoms, such as mosaic, and streak, appeared on Narcissus in Yangzhou, Jiangsu province. The previously reported narcissus virus universal pair of primers were used to perform the RT-PCR, and the results showed that the expected DNA fragments could be amplified from three samples collected from Yangzhou. Cloning, sequencing and analysis showed that the amplified sequence contained three different types, with 93%, 98% and 99% identities with the Narcissus common latent virus (NCLV), Narcissus degeneration virus (NDV) and Narcissus yellow stripe virus (NYSV), respectively. These results suggested that narcissus was infected by three viruses, NCLV, NDV and NYSV. This is the first report that the narcissus was infected by NCLV, NDV and NYSV in Jiangsu province. In the phylogenetic tree, all NCLV isolates were divided into two groups, while NCLV-YZ clustered with Shanghai isolates as one group; all NDV isolates were divided into two groups, and NDV-YZ clustered into group Ⅱ together with other Chinese isolates; all NYSV isolates were divided into four groups with highest diversity, and NYSV-YZ clustered into group Ⅰ together with some Chinese and Japanese isolates. The phylogenetic tree constructed with both NCLV and NDV rather than NYSV reflected obvious geographical specificity.
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