刘娟娟 1, 李 平 2, 江幸福 1, 程云霞 1, 张 蕾 1*.Met基因在黏虫生殖中的功能分析[J].植物保护,2019,45(6):92-97. |
Met基因在黏虫生殖中的功能分析 |
Function of methoprene-tolerant genes in the reproduction of Mythimna separata |
投稿时间:2019-03-08 修订日期:2019-04-05 |
DOI:10.16688/j.zwbh.2019113 |
中文关键词: 黏虫 保幼激素 Met 卵黄原蛋白 生殖 |
英文关键词:Mythimna separata juvenile hormone methoprene-tolerant vitellogenin reproduction |
基金项目:国家自然科学基金(31871951,31672019);国家重点研发计划(2017YFD0201802,2017YFD0201701);北京市自然科学基金(6172030);国家绿肥产业技术体系(CARS-22);国家公益性行业(农业)科研专项(201403031);中央级公益性科研业务费专项(1610142017009) |
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中文摘要: |
保幼激素(juvenile hormone, JH)是由咽侧体分泌的倍半萜类化合物, 可以调控昆虫的很多生理过程, 如发育、变态、生殖等。作为bHLH-PAS(helix-loop-helix-Per-ARNT-Sim)转录因子家族成员之一的methoprene-tolerant (Met)是JH的受体, 在JH的信号传导过程中有非常重要的作用。本研究通过实时荧光定量PCR结合RNAi技术测定了沉默Met基因后黏虫Mythimna separata的Vg基因表达、卵巢发育和生殖行为, 旨在探究Met基因在黏虫生殖过程中的功能。结果表明当Met基因沉默后, 与卵巢发育密切相关的卵黄原蛋白(vitellogenin, Vg)基因表达量下调了50%, 从而显著抑制了卵巢发育, 并导致产卵显著延迟、产卵历期显著缩短, 产卵量显著下降。结果表明Met基因是黏虫生殖发育过程中的关键受体基因, 它通过调节后续Vg的表达和沉积, 来达到控制卵巢发育, 从而调控生殖作用。 |
英文摘要: |
Juvenile hormone (JH) is a sesquiterpene compound secreted by the corpus allatum and can regulate many physiological processes of insects, such as development, metamorphosis, reproduction, etc. Methoprene-tolerant (Met), belonging to the family of the basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)transcription factors, is most likely the receptor of juvenile hormone (JH) and plays a crucial role in JH signaling pathway. In this study, we used qPCR combined with RNAi to determine the expression of Vg gene, ovarian development and reproductive behavior in M.separata after silencing Met gene, aiming to explore the functions of Met in the reproduction of M.separata. The results showed that, when Met gene was silenced, the expression of Vg gene, which is closely related to ovarian development, was down-regulated by 50%, leading to the significant inhibition of ovarian development, the delay of oviposition and the decrease of oviposition period and egg production. The results indicated that the Met gene is a key receptor gene in the process of reproductive development of M.separata. It regulates the expression and deposition of Vg to control ovarian development and regulate reproduction in M.separata. |
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