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刘础荣1, 董 玥1, 吕青阳1, 李迎宾1, 蒋 娜1, 罗来鑫1, 朱天生2, 李健强1*.防治红枣黑斑病的杀菌剂筛选和复配研究[J].植物保护,2019,45(5):263-268.
防治红枣黑斑病的杀菌剂筛选和复配研究
Screening of fungicides and their cocktail mixture forcontrolling black spot of jujube
投稿时间:2018-10-10  修订日期:2018-11-04
DOI:DOI: 10.16688/j.zwbh.2018438
中文关键词:  红枣黑斑病  链格孢菌  毒力测定  药剂复配
英文关键词:jujube black spot  Alternaria sp.  toxicity test  fungicide mixture
基金项目:国家科技支撑计划(2014BAC14B04-3); 新疆科技援疆项目(201491150); 新疆维吾尔自治区高层次人才引进项目
作者单位
刘础荣1, 董 玥1, 吕青阳1, 李迎宾1, 蒋 娜1, 罗来鑫1, 朱天生2, 李健强1* 1. 中国农业大学植物保护学院, 种子病害检验与防控北京市重点实验室, 北京 100193
2. 塔里木大学植物科学学院, 阿拉尔 843300 
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中文摘要:
      采用菌丝生长速率法和孢子萌发抑制法, 测定了14种杀菌剂单剂及基于单剂筛选结果的二元复配剂对红枣黑斑病菌Alternaria tenuissima菌丝生长和孢子萌发的抑制作用, 以此评价防治红枣黑斑病的杀菌剂和复配药剂的效果。结果表明, 咯菌腈和嘧菌环胺对靶标病菌菌丝生长的抑制效果最佳, 其EC50分别为0.091 3和0.099 8 μg/mL; 吡唑醚菌酯和啶酰菌胺对病菌孢子萌发抑制效果最佳, 其EC50分别为0.015 3和0.293 4 μg/mL。吡唑醚菌酯与戊唑醇按照8∶2和3∶7(w/w)的比例进行复配, 对病菌菌丝生长的抑制表现出相加作用, 其SR值分别为1.124 5和0.916 9; 两者以5∶5和3∶7(w/w)的比例进行复配, 对孢子萌发的抑制表现出相加作用, 其SR值分别为1.164 6和0.901 0。吡唑醚菌酯、啶酰菌胺、咯菌腈、嘧菌环胺和异菌脲等对病菌菌丝生长和孢子萌发均有抑制效果; 吡唑醚菌酯与戊唑醇3∶7(w/w)复配对病菌菌丝生长及孢子萌发的抑制效果均表现出相加作用。上述结果为红枣黑斑病防治药剂的开发和后续田间应用提供了理论支持。
英文摘要:
      In order to evaluate the effect of fungicides for controlling black spot of jujube (Ziziphus jujuba), 14 fungicides as well as their cocktail mixture were screened by measuring the mycelial growth and spore germination. The results indicated that mycelial growth of Alternaria tenuissima was strongly inhibited by fludioxonil and cyprodinil, with the EC50 values of 0.091 3 and 0.099 8 μg/mL, respectively. The spore germination was strongly inhibited by pyraclostrobin and boscalid, with the EC50 values of 0.015 3 and 0.293 4 μg/mL, respectively. The mixture of pyraclostrobin and tebuconazole had an additive effect on the inhibition of mycelial growth at the ratio of 8∶2 or 3∶7(w/w), with the synergistic ration (SR) value of 1.124 5 and 0.916 9, respectively. The mixture of these two fungicides also had an additive effect on the inhibition of spore germination at the ratio of 5∶5 or 3∶7(w/w), with the SR value of 1.164 6 and 0.901 0, respectively. In conclusion, pyraclostrobin, boscalid, fludioxonil, cyprodinil and iprodione showed inhibition effects on mycelial growth and spore germination. The mixture of pyraclostrobin and tebuconazole at the ration of 3∶7 had additive effect both on mycelial growth and spore germination, indicating that these fungicides and mixture supplied an alternative choice for jujube black spot disease control in field.
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