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王 彪1, 崔文艳1, 武君洁1, 何朋杰1, 吴毅歆1, 2, 何鹏飞1, 2, 王再强1, 李兴玉1, 2, 何月秋1, 2*.大白菜软腐病拮抗菌的筛选、鉴定及其防效研究[J].植物保护,2019,45(5):157-162.
大白菜软腐病拮抗菌的筛选、鉴定及其防效研究
Screening, identification and control effect of the antibacteriaagainst soft rot of Chinese cabbage
投稿时间:2018-09-26  修订日期:2018-11-01
DOI:DOI: 10.16688/j.zwbh.2018416
中文关键词:  拮抗菌  筛选  防效  鉴定  解淀粉芽胞杆菌
英文关键词:antagonistic bacteria  screening  control effect  identification  Bacillus amyloliquefaciens
基金项目:科技部国际合作计划项目(2009DFA32360)
作者单位
王 彪1, 崔文艳1, 武君洁1, 何朋杰1, 吴毅歆1, 2, 何鹏飞1, 2, 王再强1, 李兴玉1, 2, 何月秋1, 2* 1. 云南农业大学, 昆明 650201
2. 微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650217 
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中文摘要:
      为筛选对大白菜软腐病具有较好拮抗效果的生防菌株, 采用对峙平板法分别从作物根际土、发酵池以及本实验室菌种库得到6株拮抗性较强的菌株ASR-12、ASR-23、ASR-31、ASR-54、ASR-55、ASR-150。在离体叶柄软腐防效试验中, 采用注射接种法获得了拮抗性较强的3株拮抗菌ASR-12、ASR-23和ASR-150, 48 h的防效分别达到了87.64%、80.90%和70.79%。温室防效试验的结果表明:菌株ASR-12、ASR-23和ASR-150防治效果分别为59.22%、59.21%和55.00%, 显著高于72%农用链霉素可湿性粉剂的防效(39.15%), 具有很好应用前景。依据形态、生理生化和分子鉴定, 菌株ASR-12被鉴定为解淀粉芽胞杆菌Bacillus amyloliquefaciens。
英文摘要:
      In order to screen the antagonistic strains with good effects on soft rot of Chinese cabbage, a total of 6 strong antagonistic strains, ASR-12, ASR-23, ASR-31, ASR-54, ASR-55 and ASR-150, were isolated from the rhizosphere soil of the crops in the greenhouse, fermentation pool and bacterial bank through confrontation plate culture. Three antagonistic strains, ASR-12, ASR-23 and ASR-150, were selected for soft rot resistance test by petiole injection method of Chinese cabbage in vitro, and their control effects were 87.64%, 80.90% and 70.79%, respectively, in 48 h. The three strains were tested against soft rot in the greenhouse by spraying method, and the results showed that the control effects of the strains ASR-12, ASR-23 and ASR-150 were 59.22%, 59.21% and 55.00%, respectively, significantly better than 72% agricultural streptomycin wettable powders whose effect was 39.15%, indicating they have good application prospect. Based on morphological, physiological and biochemical and molecular identification, the strain ASR-12 was identified as Bacillus amyloliquefaciens.
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