| 张晓勇, 李树江, 王 亮, 杨友联*.山茶灰斑病病原菌鉴定及防治药剂初步筛选[J].植物保护,2019,45(4):209-215. |
| 山茶灰斑病病原菌鉴定及防治药剂初步筛选 |
| Pathogen identification of gray leaf spot on Camellia japonica andscreening of the fungicides |
| 投稿时间：2018-07-25 修订日期：2018-08-12 |
| DOI：DOI: 10.16688/j.zwbh.2018331 |
| 中文关键词: 山茶 拟盘多毛孢 灰斑病 形态特征 多基因序列分析 杀菌剂 |
| 英文关键词:Camellia japonica Pestalotiopsis gray leaf spot morphological characteristics multilocus sequence fungicide |
| 基金项目:贵州省自然科学基金(黔科合J字［2014］7447号); 贵州省特色重点实验室建设项目(黔教合KY字［2017］012); 贵州省教育厅自然科学研究项目(黔教合KY字［2015］503); 六盘水师范学院科技创新团队(LPSSYKJTD201602); 六盘水市科技局联合基金(52020-2017-02-03) |
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| 中文摘要: |
| 为明确引起贵州六盘水山茶灰斑病的致病菌种类和防治方法, 用单孢分离法获得3株菌株。通过形态学观察和rDNA-ITS、β-tubulin、tef1多基因序列进行分析, 确定该病原菌为葡萄牙拟盘多毛孢Pestalotiopsis portugalica。6种常规杀菌剂室内药效测定结果表明, 50%多菌灵可湿性粉剂和70%甲基硫菌灵可湿性粉剂两种苯并咪唑类杀菌剂在推荐浓度范围内对P.portugalica的抑制率达到了100%, 75%百菌清可湿性粉剂在推荐浓度范围内对该病原菌的抑制率也在90%以上; 有效成分浓度为250~500 mg/L的70%代森锰锌可湿性粉剂和75~300 mg/L的75%百菌清可湿性粉剂可显著促进P.portugalica产孢。 |
| 英文摘要: |
| Gray leaf spot that causes severe losses on Camellia japonica was widespread in Liupanshui city, Guizhou province. In this study, three strains (LPSU 2014016, LPSU 2014023 and LPSU 2014024) were obtained by single spore isolation from C.japonica and fungicides were screened in vitro. Based on morphological characteristics and multilocus sequence (ITS, β-tubulin and tef1) analysis, three stains were identified to be the same species, Pestalotiopsis portugalica Maharachch., K.D.Hyde & Crous. Indoor screening tests showed that two benzimidazole fungicides, carbendazim 50% WP and thiophanate-methyl 70% WP at the recommended concentrations had the inhibition rate of 100%. Chlorothalonil 75% WP at the recommended concentrations was also significantly effective with the inhibition rate over 90%. Mancozeb 70% WP at 250-500 mg/L (active ingredient) and chlorothalonil 75% WP at 75-300 mg/L could significantly induce the sporulation of P.portugalica. |
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