| 刘洪玉, 孙子豪, 李保华, 王彩霞*.侵染‘舞美’苹果的苹果锈果类病毒检测与全序列分析[J].植物保护,2019,45(4):176-179. |
| 侵染‘舞美’苹果的苹果锈果类病毒检测与全序列分析 |
| Detection and full sequence analysis of Apple scar skin viroid in‘Maypole’ apple |
| 投稿时间：2018-06-01 修订日期：2018-11-14 |
| DOI：DOI: 10.16688/j.zwbh.2018233 |
| 中文关键词: 苹果锈果类病毒 ‘舞美’ RT-PCR 序列分析 |
| 英文关键词:Apple scar skin viroid ‘Maypole’ RT-PCR sequence analysis |
| 基金项目:国家现代农业(苹果)产业技术体系(CARS-28); 青岛农业大学大学生创新训练项目 |
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| 中文摘要: |
| 2017年9月, 在山东胶州调查苹果病毒病时发现‘舞美’果实表现出明显的花脸症状, 取发病树体芽组织嫁接于健康‘富士’苹果幼树上, 采用RT-PCR技术对其是否含有苹果锈果类病毒进行了检测, 并利用DNAMAN、MEGA 5.1等软件对其全序列进行了分析。结果表明, 显症‘舞美’果实、发病树体枝条及嫁接‘富士’枝条中均可检测出ASSVd, 无症状‘舞美’果实和相应树体枝条中均未检测到ASSVd。‘舞美’分离物基因组主流序列为333 nt (登录号MG745387), 与GenBank中已报道的ASSVd序列一致性为92%~99%。序列多重比对及系统进化树分析发现, 该分离物的末端保守区和中央保守区与ASSVd参考序列一致, 且与不同来源的ASSVd分离物亲缘关系较近。这是首次在‘舞美’苹果上发现和鉴定出苹果锈果类病毒。 |
| 英文摘要: |
| In September 2017, ‘Maypole’ fruits with typical dappling and scaring symptoms were found during the investigation of apple virus diseases in Jiaozhou, Shandong. The buds from diseased ‘Maypole’ tree were grafted on the healthy ‘Fuji’ apple tree. Apple scar skin viroid (ASSVd) was detected by using RT-PCR method. The full nucleotide sequence of the isolate was analyzed by DNAMAN and MEGA 5.1 software. The results showed that ASSVd was detected in the symptomatic ‘Maypole’ fruits, branches from the tree with diseased fruits and ‘Fuji’ apple tree grafted with diseased buds, while no ASSVd was detected in the asymptomatic ‘Maypole’ fruits and branches in the corresponding tree. The dominant sequences of ‘Maypole’ isolate comprised 333 nucleotides (GenBank accession no. MG745387), which shared 92% to 99% identity with other ASSVd sequences registered in the GenBank. Multiple alignment and phylogenetic tree analysis of ASSVd nucleotide sequences revealed that the terminal conserved region (TCR) and central conserved region (CCR) of this isolate were identical with those of the reported reference sequences. In addition, the ‘Maypole’ isolate was closely related with other ASSVd isolates from different sources. For the first time, ASSVd was found and identified in ‘Maypole’. |
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