| 李苗苗1, 3, 伍 翔4, 严 准1, 吴中军2, 3*, 高春生1, 刘新宇5, 程 毅1*.一株强致病力软腐病菌鉴定与室内药剂筛选[J].植物保护,2019,45(4):149-155. |
| 一株强致病力软腐病菌鉴定与室内药剂筛选 |
| Identification of a strong pathogen causing soft rot and indoorscreening for bactericides |
| 投稿时间：2018-08-22 修订日期：2018-09-22 |
| DOI：DOI: 10.16688/j.zwbh.2018365 |
| 中文关键词: 软腐病菌 看家基因 迪基氏菌属 MLSA 最小抑菌浓度 |
| 英文关键词:soft rot pathogen housekeeping genes Dickeya MLSA minimal inhibit concentration |
| 基金项目:中央级公益性科研院所基本科研业务费专项资金(1610242016025); 中国农业科学院科技创新工程项目(CAAS-ASTIP-2015-IBFC09); 湖南省自然科学基金项目(2017JJ3351); 湖南省重点研发计划(2016WK2030) |
| 作者 | 单位 | | 李苗苗1, 3, 伍 翔4, 严 准1, 吴中军2, 3*, 高春生1, 刘新宇5, 程 毅1* | 1. 中国农业科学院麻类研究所, 南方经济作物研究中心, 长沙 410205
2. 重庆文理学院林学与生命科学学院, 重庆 402160
3. 西南大学园艺园林学院, 重庆 400715
4. 北京图为生物科技有限公司, 北京 100096
5. 河北北方学院植物保护系, 张家口 075000 |
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| 中文摘要: |
| 通过对从湘西武陵山区感病萝卜分离的一株软腐病菌进行致病力测定, 结合萝卜软腐病菌的培养特性、菌体形态观察、生理生化测定及6个看家基因(gapA, icdA, pgi, rpoS, mdh, proA)的多位点序列分析(MLSA), 结果表明，病原细菌Secpp 1600属革兰氏阴性菌, 呈杆状, 致病力强, 可侵染分属8科13个属的多种经济作物; 与迪基氏菌Dickeya fangzhongdai菌株高度同源。选用7种药剂通过抑菌圈法对Secpp 1600进行了抑菌效果初步测定, 结果表明, 农用链霉素、SPP3和SPP4 可抑制病菌生长, 药剂间抑菌率存在显著差异, 其中, SPP4的直接抑菌效果最好, 最小抑制浓度为900 μg/mL。 |
| 英文摘要: |
| The pathogenicity of a pathogen isolated from the radish samples with the typical symptoms of soft rot in the Wuling Mountain in western Hunan was studied, and then the strain was identified by cultural and morphological characteristics, physicochemical tests, multilocus sequence analysis (MLSA) of 6 housekeeping genes (gapA, icdA, pgi, rpoS, mdh, proA). The results showed that the bacterial pathogen was Gram-negative, rod-shaped with strong pathogenicity of soft rot and infected various economic crops belonging to 13 genera in 8 families. The results showed that the strain shared a high similarity with Dickeya fangzhongdai. We determined the toxicity of 7 bactericides by the inhibition zone method. The results showed that agricultural streptomycin, SPP3 and SPP4 inhibited the growth of Secpp 1600 and there were significant differences among them in the inhibition rate. SPP4 showed the best direct antibacterial effect and the minimal inhibit concentration was 900 μg/mL. |
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