| 黄兆峰1, 白薇薇2, 周欣欣3, 黄红娟1, 姜翠兰1, 张朝贤1, 魏守辉1*.田旋花EPSPS基因表达特征及草甘膦对其表达的影响[J].植物保护,2019,45(4):104-107. |
| 田旋花EPSPS基因表达特征及草甘膦对其表达的影响 |
| Expression pattern of EPSPS gene and the effect of glyphosate on itsexpression in field bindweed |
| 投稿时间：2018-08-30 修订日期：2018-10-09 |
| DOI：DOI: 10.16688/j.zwbh.2018372 |
| 中文关键词: 田旋花 草甘膦 EPSPS 表达 |
| 英文关键词:Convolvulus arvensis glyphosate EPSPS expression |
| 基金项目:国家自然科学基金(31501659); 国家重点研发计划(2018YFD0200602); 农业部西北荒漠绿洲作物有害生物综合治理重点实验室(KFJJ20180104) |
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| 中文摘要: |
| 采用实时荧光定量RT-PCR测定了田旋花不同组织、不同叶龄的EPSPS基因mRNA的相对表达量以及草甘膦对EPSPS基因相对表达量的影响。结果表明: 田旋花EPSPS基因在不同组织表达量差异显著, 在叶的表达量高于茎和根; 该基因在9叶期的表达量最高, 是3叶期的1.5倍; 在草甘膦处理后, 田旋花EPSPS基因的表达量先升高后降低, 在处理后24 h达最大值。随草甘膦剂量增加, 该基因的表达量升高。研究结果可为深入解析田旋花对草甘膦耐药性机理提供参考。 |
| 英文摘要: |
| Quantitative RT-PCR was employed to analyze the expression pattern of EPSPS gene in different tissues of the field bindweed Convolvulus arvensis at different leaf stages and the effect of glyphosate on the EPSPS gene expression. The results showed that the expression of EPSPS in leaves was higher than in roots and stems. The highest EPSPS expression level was observed at 9-leaf stage, 1.5 times as that at 3-leaf stage. After glyphosate treatment, EPSPS expression level increased first and then decreased, and the peak induction was detected at 24 h after glyphosate application. The EPSPS expression level was enhanced with increasing glyphosate dose. The results provide a reference for illuminating the mechanism of glyphosate tolerance in field bindweed. |
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