| 杨小龙1,2, 陈细红2, 蔡 伟3, 高芳銮1, 沈建国2, 吴祖建1*.福建省福清地区马铃薯病毒病病原的分子检测[J].植物保护,2019,45(3):201-205. |
| 福建省福清地区马铃薯病毒病病原的分子检测 |
| Molecular detection of potato virus pathogens in Fuqing area ofFujian province |
| 投稿时间：2018-03-01 修订日期：2018-07-29 |
| DOI：10.16688/j.zwbh.2018095 |
| 中文关键词: 福清地区 马铃薯病毒 RT PCR 检测与鉴定 |
| 英文关键词:Fuqing area potato virus RT PCR detection and identification |
| 基金项目:国家重点研发计划(2016YFF0203203); 福州市科技计划(2013-N-54) |
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| 中文摘要: |
| 2017年调查福建福清地区马铃薯病毒病的发生情况, 以明确该地区马铃薯主要病毒病原。共采集了46份疑似感染病毒的马铃薯植株, 提取总RNA, 利用RT PCR技术进行分子检测, 结果表明, 福清地区危害马铃薯的病毒有马铃薯Y病毒Potato virus Y(PVY)、马铃薯卷叶病毒Potato leaf roll virus(PLRV)、马铃薯S病毒Potato virus S(PVS), 检出率分别为56.52%、17.39%和10.87%, 以PVY检出率最高, 说明PVY是危害该地区马铃薯样品的主要病毒病原。通过病毒复合侵染进行分析, 发现该地区存在病毒复合侵染马铃薯现象。研究结果可为福清地区马铃薯种薯的引进和病毒病害防治提供参考依据。 |
| 英文摘要: |
| The aim of this study is to investigate the occurrence of potato viruses in Fuqing area, Fujian province, and to diagnose the main viruses causing major diseases. In 2017, 46 infected potato plant samples were collected from Fuqing area, and the total RNA was extracted from the infected potato leaves. From these samples, PVY, PLRV and PVS were detected via the conventional RT PCR techniques, and the detection rates were 56.52%, 17.39% and 10.87%, respectively, while other viruses were not detected. The analysis of complex infection showed that complex infection existed in potato plants in this area. This study can provide reference for the introduction of seed potato and the management strategy of virus diseases control in Fuqing area. |
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