| 曾耿狄, 符可芯, 刘 乐, 余 东, 王 靖, 冼欣唯, 杨 叶*.海南芒果可可球二孢的致病力及其对多菌灵抗性[J].植物保护,2019,45(3):174-178. |
| 海南芒果可可球二孢的致病力及其对多菌灵抗性 |
| Virulence and carbendazim resistance of Botryodiplodiatheobromae causing mango stem end rot in Hainan |
| 投稿时间：2018-12-04 修订日期：2019-01-09 |
| DOI：10.16688/j.zwbh.2018498 |
| 中文关键词: 芒果 可可球二孢 致病力 多菌灵 抗药性 |
| 英文关键词:mango Botryodiplodia theobromae virulence carbendazim fungicide resistance |
| 基金项目:国家自然科学基金(31560521) |
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| 中文摘要: |
| 从海南5个芒果主产区采集分离芒果蒂腐病菌可可球二孢菌株, 采用室内人工接种的方法对110个可可球二孢菌株进行致病力测定, 采用区分剂量法和菌丝生长速率法测定其中106个菌株对多菌灵的抗性。结果表明:芒果蒂腐病自然发病率29.37%; 人工接种的发病率100.00%, 110株可可球二孢菌株中强致病力、中等致病力及弱致病力菌株分别占64.55%、25.45%和10.00%; 在106株可可球二孢菌株中, 抗性菌株发生频率为52.83%, 其中高抗菌株可在含100 mg/L多菌灵培养基上生长, 发生频率为45.28%, 中抗菌株在含100 mg/L多菌灵培养基上不能正常生长, 抗性频率为7.55%; 没有发现低抗菌株。多菌灵对可可球二孢田间分离菌株EC50的频率分布为不连续分布, 研究结果表明海南地区危害芒果的可可球二孢对多菌灵抗性非常严重。 |
| 英文摘要: |
| The strains of Botryodiplodia theobromae were collected and separated from mango infected by stem end rot in five main mango producing areas of Hainan. The virulence of 110 B. theobromae strains were determined by the artificial inoculation. The resistance of 106 strains to carbendazim were tested by distinguishing dosage method and mycelial growth rate method. The results showed that the incidence of mango stem end rot was 29.37% and 100% under natural and artificial inoculation conditions, respectively. In total of 110 strains, the high virulent strains, median virulent strains, and the low virulent strains accounted by 64.55%, 25.45% and 10.00%, respectively. The resistance frequency of 106 strains to carbendazim was 52.83%. Among them, high resistance strains could grow on media containing 100 mg/L carbendazim with the resistance frequency of 45.28% , the median resistance strains not grow normally on the media containing 100 mg/L carbendazim with the resistance frequency of 7.55%, and no low resistance strains were found. The carbendazim sensitivity frequency showed non continuous distribution. All data showed that B. theobromae from mango had serious resistance to carbendazim in Hainan. |
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