| 罗 宁1, 李惠霞1*, 郭 静1, 徐鹏刚1, 张淑玲1, 刘永刚2.甘肃省陇东南大豆孢囊线虫的发生和分布[J].植物保护,2019,45(3):165-169. |
| 甘肃省陇东南大豆孢囊线虫的发生和分布 |
| Occurrence and distribution of Heterodera glycines insoutheast of Gansu province |
| 投稿时间：2018-06-12 修订日期：2018-09-01 |
| DOI：10.16688/j.zwbh.2018253 |
| 中文关键词: 大豆孢囊线虫病 分布 发生 鉴定 |
| 英文关键词:soybean cyst nematode disease distribution occurrence identification |
| 基金项目:国家公益性行业(农业)科研专项(201503114); 甘肃省科技支撑计划(1604NKCA053-2) |
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| 中文摘要: |
| 为了解甘肃省陇东南地区大豆孢囊线虫病分布和发生程度, 采用Z字形取样法, 对甘肃省陇东南地区9个县(区)37个乡镇大豆孢囊线虫病的发生和分布进行调查, 并根据形态特征和rDNA ITS序列比对鉴定线虫种类。共采集土样411份, 陇东地区大豆孢囊线虫检出率为87.2%, 其中镇原、西峰、正宁、华池和环县的检出率最高, 达100%, 镇原孢囊发生量最大, 平均孢囊数为11.4个/100 g土; 陇南地区大豆孢囊线虫检出率为63.7%, 成县和徽县分别为64.0%、63.0%, 平均孢囊数分别为0.9和1.2个/100 g土。经形态学特征观测和rDNA ITS序列比对, 甘肃省陇东南采集到的孢囊线虫均鉴定为大豆孢囊线虫Heterodera glycines。 |
| 英文摘要: |
| In order to understand the occurrence and distribution of soybean cyst nematode in southeast of Gansu Province, zigzag sampling method was used to investigate soybean cyst nematodes in 37 townships of 9 counties in southeastern Gansu. The species of nematodes were identified based on the morphological observation and rDNA-ITS sequence analysis. Totally, 411 samples of soil samples were collected. The results showed that the detection rate of soybean cyst nematodes in eastern Gansu was 87.2%, among which the detection rates in Zhenyuan, Xifeng, Zhengning, Huachi and Huan County were the highest, with the rates of 100%, while that in Qingcheng was the lowest by 39.3%. The highest density was found in Zhenyuan with the average cysts number of 11.4 per 100 g soil. The detection rate of soybean cyst nematodes in southern Gansu was 63.7% and those in Chengxian and Huixian were 64.0% and 63.0%, respectively, and average densities of nematodes were 0.9 and 1.2 per 100 g soil, respectively. Morphological observation and rDNA-ITS sequence analysis showed that the cyst nematodes collected in southeastern Gansu were identified as Heterodera glycines. |
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