| 宋晓宇1, 刘 勇1,2, 陈建斌1, 史晓斌1,2*, 张德咏1,2*.番茄斑萎病毒系统侵染我国大蒜[J].植物保护,2019,45(3):149-151. |
| 番茄斑萎病毒系统侵染我国大蒜 |
| Systemic infection of Chinese garlic by Tomato spotted wilt tospovirus |
| 投稿时间：2018-06-21 修订日期：2018-08-02 |
| DOI：10.16688/j.zwbh.2018266 |
| 中文关键词: 番茄斑萎病毒 大蒜 鉴定 RT-PCR |
| 英文关键词:Tomato spotted wilt tospovirus garlic identification RT-PCR |
| 基金项目:湖南省科技计划项目(2016RS2019); 国家自然科学基金(31571981, 31571982) |
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| 中文摘要: |
| 番茄斑萎病毒Tomato spotted wilt tospovirus(TSWV)是严重危害世界经济作物的一种病毒, 寄主范围广泛。我们在研究中发现番茄斑萎病毒能侵染我国大蒜Allium sativum L.。利用摩擦接种法将TSWV接种到健康大蒜上, 结果显示:接种14 d后大蒜新生叶片出现褪绿和白色斑点症状。ELISA检测显示大蒜叶片汁液与TSWV的单克隆抗体产生血清学反应, 采用TSWV N基因的引物对大蒜叶片总RNA进行RT PCR, 结果扩增出约800 bp的条带, 在NCBI上BLAST显示与TSWV YN5576的同源性最高, 为98.52%。这些数据表明番茄斑萎病毒系统侵染我国大蒜。 |
| 英文摘要: |
| Tomato spotted wilt tospovirus (TSWV) causes severe damages to economic crops worldwide, and it can infect a wide range of plant hosts. To determine whether TSWV infect Chinese garlic, the extracts of Nicotiana benthamiana leaves that were infected with TSWV were rub inoculated to garlic plants. Garlic plants showed chlorotic and white spot symptoms on new leaves at 14 d post inoculation. Then the garlic leaves were detected by ELISA and RT-PCR. Positive results were obtained in the Dot ELISA of the garlic leaf extracts with monoclonal antibody of TSWV. Furthermore, the target fragments of 800 bp were obtained from all infected garlic leaves by RT-PCR using the specific primers for N gene of TSWV. The sequence of the RT-PCR products from infected garlic leave was analyzed at NCBI, and the results showed that it shared 98.52% of nucleic acids with TSWV YN5576 isolate. Therefore, our results showed that Chinese garlic could be infected by TSWV. |
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