| 马永强, 朱海霞*.具有除草活性的生防菌株GD-9发酵条件优化及菌剂制备[J].植物保护,2019,45(3):88-95. |
| 具有除草活性的生防菌株GD-9发酵条件优化及菌剂制备 |
| Optimization of fermentation conditions and preparation of microbialinoculum for biocontrol strain GD 9 with herbicidal activity |
| 投稿时间：2018-06-12 修订日期：2018-08-13 |
| DOI：10.16688/j.zwbh.2018255 |
| 中文关键词: 生防菌株 除草活性 发酵条件 菌剂制备 |
| 英文关键词:biocontrol strain herbicidal activity fermentation conditions preparation of microbial inoculum |
| 基金项目:国家自然科学基金(31560518); 中国科学院“西部之光”人才培养引进计划(201523) |
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| 中文摘要: |
| 为了明确具有除草活性的生防菌株GD-9发酵过程中各因子的配比和最优条件, 采用单因素试验对菌株最适碳源、氮源、载体、固态发酵基质进行了筛选, 应用正交试验设计研究了碳源、氮源、初始含水量、接种量、初始pH、培养时间、培养温度7种因素对菌株活菌数的影响。试验结果表明菌株GD-9最佳固态发酵条件为:氮源NaNO3 58.4 mg/g, 碳源葡萄糖48.4 mg/g, 培养基质最适初始含水量为258 mg/g, 最适接种量为0.26 mL/g, 初始 pH 7.6, 最佳培养时间147.8 h, 最佳培养温度30.7℃, 最适宜的载体为黏土, 分散剂为聚乙烯醇, 稳定剂为膨润土, 润湿剂为糊精。通过发酵试验结果制备生防菌株GD-9的固体菌剂, 该研究为菌剂的商品化生产奠定基础。 |
| 英文摘要: |
| In order to clarify the proportion and optimal conditions of various factors in the fermentation process of biocontrol strain GD-9 with herbicidal activity, the single factor experiment was performed to screen the optimal carbon source, nitrogen source, carrier and solid fermentation substrate of the strain. The orthogonal experiment design was used to study the influences of carbon source, nitrogen source, initial water content, inoculation amount, initial pH value, culture time and culture temperature on the colony number of the strain. The experimental results showed that the optimal solid state fermentation conditions of strain GD-9 were N source NaNO3 58.4 mg/g, C source glucose 48.4 mg/g, 258 mg/g of initial water content of the culture medium, 0.26 mL/g of inoculation amount, the initial pH 7.6, 147.8 h and 30.7℃ of culture time and temperature, respectively. The optimal carrier, dispersant, stabilizer and wetting agent were clay, polyvinyl alcohol, bentonite and dextrin. The solid microbial inoculum of biocontrol strain GD-9 was prepared through fermentation test results. This study laid a foundation for the commercial production of the microbial inoculum. |
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