| 潘鹏亮, 张方梅, 尹 健, 刘红敏*, 史洪中.翅形态特征在重阳木斑蛾成虫性别鉴定中的应用[J].植物保护,2019,45(3):71-77. |
| 翅形态特征在重阳木斑蛾成虫性别鉴定中的应用 |
| Application of wing morphological characteristics in the sex identification ofHistia rhodope Cramer (Lepidoptera: Zygaenidae) adults |
| 投稿时间：2018-07-14 修订日期：2018-08-07 |
| DOI：10.16688/j.zwbh.2018310 |
| 中文关键词: 重阳木斑蛾 数值分类 特征提取 翅脉交点 雌雄判别 |
| 英文关键词:Histia rhodope numerical taxonomy feature extraction vein junction sex identification |
| 基金项目:河南省高等教育教学改革研究与实践项目(2017SJGLX135); 信阳农林学院重点学科培育学科建设项目(ZDXK201701); 河南省高等学校重点科研项目(19A210021) |
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| 中文摘要: |
| 为了明确重阳木斑蛾的翅形态特征在其性别鉴定中的作用, 探索雌雄难于区分的成虫的数值鉴定方法。采用图像处理与分析技术, 通过爱普生扫描仪获取重阳木斑蛾前翅图, 利用BugShape v 1.0软件提取雌雄成虫翅的轮廓特征, 利用tpsDig 2软件获取翅脉交叉点位置特征, 并利用独立样本t检验和标志点空间普氏叠加和扭曲分析, 以明确此类特征在该成虫雌雄鉴定中的作用。结果表明, 重阳木斑蛾成虫左右前翅轮廓特征在同一性别中差异不显著。仅利用左前翅数据进行分析时, 翅面积、短轴长度、等效圆半径、偏心率、球状性和圆形度在两个性别中存在极显著差异, 周长和长轴长度的差异达到显著水平, 而紧凑度和叶状性差异不显著。使用左右前翅作为数据集进行分析时, 除紧凑度和叶状性差异仍不显著外, 其余参数均达到极显著水平。雌雄前翅轮廓特征作为指标来判别性别时正确率为100%, 但存在左右翅相互错判现象。翅膀内部翅脉交叉点的空间位置在个体间存在较大差异, 主要表现在相对于翅的横向方向变异大。雌雄间的差异主要存在于外缘附近的翅脉交叉点。结论:通过前翅的轮廓特征可以区分重阳木斑蛾雌雄成虫的差异。翅脉交叉点空间位置在雌雄间和个体间存在一定的变异。 |
| 英文摘要: |
| Picture processing and analysis technology was employed to clarify the effects of wing morphological characteristics of Histia rhodope adults on sex recognition and explore the numerical identification method for these adults whose sex is hard to recognize. Forewing images of H. rhodope were captured by flat plate scanner (Epson Perfection V370 Photo) with transparent adapter. The contour feature of wings from female and male adults was extracted by the software BugShape v 1.0, and the landmarks of vein junctions were acquired using tpsDig 2. Independent sample t test, Procrustes superimposition of the landmarks and relative warps were computed to verify the effects of the parameters on sex identification. The results showed that there was no significant difference in contour features between two side wings from the same sexual cases. Data analysis based on the left wings showed that the most parameters, such as area, short axis length, equalradius, eccentricity, sphericity and circularity, exhibited highly significant differences (α=0.01), or significant difference (α=0.05) in terms of perimeter and long axis length, but no significant difference existed in compactness and lobation between male and female samples. However, except for the two parameters compactness and lobation, the difference was highly significant (α=0.01) in the other parameters between male and female adults. Although some samples were not assigned to right groups between left and right wings, the accuracy distinguishing males and females was up to 100% by using forewing outline features as indicators. The spatial positions of vein landmarks showed remarkable variation, especially in transverse direction of wings, between male or female samples. There was wide variation between female and male adults in the vein landmark positions nearby the outer margin of wings. It is concluded that the difference between male and female H. rhodope adults can be distinguished by using the contour feature of fore wings. Some variation exists among the sexes and samples in the spatial position of the vein landmarks. |
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