| 罗育发1,2, 吴 凯1, 付 丹1, 王艾平1, 徐 兵1.江西亚洲柑橘木虱的种群遗传结构研究[J].植物保护,2019,45(3):55-59. |
| 江西亚洲柑橘木虱的种群遗传结构研究 |
| Population genetic structure of the Asian citrus psyllid, Diaphorina citriKuwayama (Hemiptera: Liviidae) in Jiangxi |
| 投稿时间：2018-06-04 修订日期：2018-08-14 |
| DOI：10.16688/j.zwbh.2018234 |
| 中文关键词: 亚洲柑橘木虱 柑橘黄龙病 种群遗传结构 COⅠ基因 江西 |
| 英文关键词:Diaphorina citri huanglongbing population genetic structure COⅠ gene Jiangxi |
| 基金项目:江西省高等学校科技落地计划(KJLD14081); 江西省重点研发计划(20161BBF60076); 江西省教育厅科技计划(GJJ170925，GJJ180864) |
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| 中文摘要: |
| 亚洲柑橘木虱Diaphorina citri Kuwayama是芸香科Rutaceae植物新梢期的重要害虫, 也是黄龙病Huanglongbing的主要传播媒介, 严重威胁江西柑橘产业的发展。本文利用线粒体基因标记, 研究江西10个地点亚洲柑橘木虱的种群遗传结构和多态性。扩增了2个线粒体COⅠ基因片段, 长度为1 431 bp 。其中多态性位点2个(均为非同义突变, A/G转换), 单倍型3个(Hap 1, Hap 2和Hap 3)。最常见的单倍型是Hap 1(占总样本数的91.7%), 发生在所有采样点; 其次为Hap 3(6.3%), 分布在大余和崇义; Hap 2(2.0%)仅在兴国出现。单一直线型的单倍型网络结构模式、小而不显著的HR(Harpending’s raggedness)指数值, 以及单峰型错配分布曲线均表明:江西地区的亚洲柑橘木虱是近期入侵并经历了快速的种群动态扩张。 |
| 英文摘要: |
| The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), an important pest of the young developing leaves of Rutaceae, is the main vector of the bacterium that causes citrus greening disease (huanglongbing). The population genetic structure and polymorphism of D. citri from 10 sites in Jiangxi were studied by using mitochondrial markers. Two fragments of mitochondrial COⅠ sequences were amplified with a total length of 1 431 bp. We found 2 polymorphic sites (nonsynonymous, A/G transition) and 3 haplotypes (Hap 1, Hap 2 and Hap 3). Among these, the most common haplotype was Hap 1 (91.7% of the total) occurring at all sampling sites. Hap 3 (6.3%) was distributed in Dayu and Chongyi. Hap 2 (2%) was observed only in Xingguo. The single line haplotype network model, the small and insignificant Harpending’s raggedness (HR) index and the unimodal mismatch distribution curve indicated that D. citri populations in Jiangxi had the recent invasion and rapid dynamic expansion. |
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