| 何发林1, 2, 姜兴印1, 2*, 尚佃龙1, 2, 姚晨涛1, 2, 谭海丽1, 2, 李向东1, 张吉旺3.溴氰虫酰胺胁迫对小地老虎保护酶和解毒酶活性的诱导效应[J].植物保护,2019,45(2):90-96. |
| 溴氰虫酰胺胁迫对小地老虎保护酶和解毒酶活性的诱导效应 |
| Induction of the protective and detoxification enzymes by cyantraniliprole in Agrotis ipsilon |
| 投稿时间：2018-07-04 修订日期：2018-09-11 |
| DOI：10.16688/j.zwbh.2018284 |
| 中文关键词: 溴氰虫酰胺 小地老虎 毒力 解毒酶 保护酶 |
| 英文关键词:cyantraniliprole Agrotis ipsilon toxicity detoxifying enzymes protective enzymes |
| 基金项目:国家重点研发计划(2018YFD0200604); 山东省现代农业产业技术体系项目(SDAIT-02-10); 山东“双一流”奖补项目(SYL2017-XTTD11); 山东省农业重大应用技术创新项目 |
| 作者 | 单位 | | 何发林1, 2, 姜兴印1, 2*, 尚佃龙1, 2, 姚晨涛1, 2, 谭海丽1, 2, 李向东1, 张吉旺3 | 1. 山东农业大学植物保护学院, 泰安 271018
2. 山东省农药毒理与应用技术重点实验室, 泰安 271018
3. 山东农业大学农学院, 泰安 271018 |
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| 中文摘要: |
| 为明确小地老虎对溴氰虫酰胺的代谢解毒机制, 采用浸叶法测定了溴氰虫酰胺对小地老虎3龄幼虫室内毒力, 在此基础上, 通过酶动力学法检测了溴氰虫酰胺亚致死剂量对小地老虎保护酶和解毒酶活力的影响, 并比较了不同剂量处理后1、6、12、24、48和72 h时小地老虎体内保护酶和解毒酶活力的动态变化。结果表明, 溴氰虫酰胺亚致死剂量初期可诱导过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活力升高; 对羧酸酯酶(CarE)活力表现为早期诱导、后期抑制的作用; 对谷胱甘肽S-转移酶(GSTs)活力表现为明显的诱导作用; 对多功能氧化酶(MFOs)表现为一定程度的抑制作用。研究结果为深入了解小地老虎对溴氰虫酰胺的防御机理提供了一定的理论基础。 |
| 英文摘要: |
| In order to clarify the mechanisms of metabolism and detoxification of Agrotis ipsilon after exposure to sublethal dose of cyantraniliprole, the leaf dipping method was used to determine the toxicity of cyantraniliprole against the 3rd-instar larvae of A.ipsilon in the laboratory. The effects of sublethal dose of cyantraniliprole on the activity of protective enzymes and detoxification enzymes in A.ipsilon were detected by enzyme kinetic method, and the dynamic changes of the enzyme activities were compared at 1, 6, 12, 24, 48 and 72 h, respectively. The results showed that the activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) were increased after exposed to sublethal doses of cyantraniliprole at the early stage of treatments, and the activity of carboxyl esterase (CarE) was induced at the early stage and inhibited at the later stage; the activity of glutathione S-transferase (GSTs) was significantly induced, and the activity of mixed function oxidase was, to some extent, inhibited after exposed to sublethal concentrations of cyantraniliprole. The results provide the theoretical basis for further understanding the defense mechanisms of A. ipsilon against cyantraniliprole. |
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