| 何倩倩, 刘雨欣, 方馨玫, 朱天辉, 谯天敏, 韩 珊, 李姝江*.杂交竹抗梢枯病诱导因子筛选及其持续期研究[J].植物保护,2019,45(2):81-89. |
| 杂交竹抗梢枯病诱导因子筛选及其持续期研究 |
| Screening of inducing factors of Bambusa pervariabilis×Dendrocalamopisis grandis against Arthrinium phaeospermum and their duration |
| 投稿时间：2018-01-30 修订日期：2018-03-25 |
| DOI：10.16688/j.zwbh.2018053 |
| 中文关键词: 杂交竹梢枯病 暗孢节菱孢菌 诱导因子 抗病性 孢子萌发 |
| 英文关键词:B. pervariabilis×D. grandis blight Arthrinium phaeospermum inducing factor resistance spore germination |
| 基金项目:中国博士后基金（2016M602705） |
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| 中文摘要: |
| 本文利用病原毒素同源异质的特点诱导杂交竹抗病潜力, 筛选出抗梢枯病的最佳诱导因子并排除其对病原菌的直接作用, 经琼脂玻片萌发法测定3种诱导因子(温度灭活毒素、蛋白酶降解毒素和细胞壁成分)对杂交竹梢枯病病原菌暗孢节菱孢菌孢子萌发的抑制作用, 结果显示经过60℃灭活毒素浓度为40 μg/mL处理的孢子萌发效果最为理想。通过最佳诱导因子对杂交竹不同品种诱导持续期进行测定, 采用针刺法先接种诱导因子后挑战接种病原菌, 1~40 d内观察抗感品种对诱导因子响应的差异, 症状上杂交竹8#的感病程度重于3#和6#, 40 d时叶片和枝干变黄干枯, 病情指数结果表明, 3个杂交竹品种接种诱导因子后感病程度降低, 诱抗效果显示杂交竹6#的诱抗指数高于3#和8#。以上结果证明诱导因子使不同杂交竹品种均产生了一定的抗性且抗性越强的品种诱导抗性越好。 |
| 英文摘要: |
| By the homologous heterogeneity of pathogenic toxins, the resistance potential of Bambusa pervariabilis×Dendrocalamopisis grandis was induced; the optimum inducing factor was screened and the direct effect was excluded. The inhibitory effects of three factors (inactivated toxin by temperature, degraded toxin by protease and cell wall component) on spore germination of Arthrinium phaeospermum, the pathogen of B. pervariabilis×D. grandis blight, were determined by agar slide germination method. The results showed that the spore germination effect of the toxin treated at 60℃ at the concentration of 40 μg/mL was the best. The induction durations of different varieties of B. pervariabilis×D. grandis were determined through the optimum inducing factor; the needling method was used to inoculate the inducing factor first and then the pathogen within 40 days to observe the response difference between the resistant and susceptible varieties to the inducing factor. The results showed that B. pervariabilis×D. grandis #8 was more susceptible than #3 and #6, with the yellowish and withered leaves and branches at 40 d. The disease indices of the three varieties were decreased after inoculation of the inducing factor, and the induced resistance effect of B. pervariabilis×D. grandis #6 was higher than those of #3 and #8. These results indicated that the inducing factor could induce certain resistance in different varieties of B. pervariabilis×D. grandis, and the stronger the original resistance, the better the induced resistance. |
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