| 陈利军1, 2*, 智亚楠1, 2, 陈思宇1, 马宇明1, 黄草娜1.留兰香挥发油熏蒸抑菌活性研究[J].植物保护,2019,45(2):75-80. |
| 留兰香挥发油熏蒸抑菌活性研究 |
| Fumigant antifungal activities of essential oils from Mentha spicata |
| 投稿时间：2018-04-23 修订日期：2018-05-16 |
| DOI：10.16688/j.zwbh.2018176 |
| 中文关键词: 留兰香 熏蒸抑菌活性 挥发油 抑菌机制 L-香芹酮 |
| 英文关键词:Mentha spicata fumigation antifungal activity essential oil antifungal mechanism L-carvone |
| 基金项目:国家自然科学基金（31401798）；河南省自然科学基金（182300410074） |
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| 中文摘要: |
| 为了确定留兰香挥发油对植物病原真菌的生防效果, 进一步探讨其抑菌机制, 以明确其在植物真菌病害防控方面的应用前景, 本研究采用菌丝生长速率法测定了留兰香挥发油对番茄灰霉病菌Botrytis cinerea、小麦赤霉病菌Fusarium graminearum、水稻纹枯病菌Rhizoctonia solani和莴苣菌核病菌Sclerotinia sclerotiorum 4种植物病原真菌的熏蒸抑制作用, 并利用GC-MS分析了留兰香挥发油的化学组分。结果显示, 留兰香挥发油对4种供试真菌均有强烈的熏蒸抑制作用, EC50均低于7.0 μL/L。挥发油处理后番茄灰霉病菌菌丝畸变、表面塌陷, 细胞膜被破坏、原生质泄漏, 细胞质结构呈消解状, 线粒体受损。通过GC-MS从留兰香挥发油中分离出30个组分, 鉴定出其中24个组分, 占挥发油总量的94.918%, 其主要成分有L-香芹酮(57.468%)、D-柠檬烯(8.976%)等。研究表明, 留兰香挥发油是理想的绿色熏蒸杀菌资源。 |
| 英文摘要: |
| The biocontrol potential of essential oils from Mentha spicata against plant pathogenic fungi was investigated to explore their applications in the prevention and control of plant fungal diseases. In this study, the fumigant antifungal activities of essential oils from M. spicata against four plant pathogenic fungi including Botrytis cinerea, Fusarium graminearum, Rhizoctonia solani and Sclerotinia sclerotiorum were determined by mycelial growth rate method; the influences of essential oils on mycelia of B.cinerea were observed, and chemical constituents of essential oils were analyzed by GC-MS.The results showed that the essential oils from M. spicata had strong fumigation activities against all tested pathogenic fungi and EC50 values were lower than 7.0 μL/L. After treatment with essential oils, aberration and surface depression in mycelia of B.cinerea, cell membrane destruction and cytoplasmic exosmosis, cytoplasmic structure digestion, and mitochondrial damage were observed. Totally 30 constituents were isolated, and 24 were identified by GC-MS, accounting for 94.918% of the total essential oils. The main chemical constituents included L-carvone (57.468%), D-limonene (8.976%) and so on. In conclusion, the essential oils from M. spicata were ideal green fumigant antifungal resources. |
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