| 韩 帅, 张河庆, 吴 婕, 李洪浩, 席亚东*.四川省山药黑斑病病原菌的鉴定[J].植物保护,2019,45(2):68-74. |
| 四川省山药黑斑病病原菌的鉴定 |
| Identification of the pathogen causing black rot of yam in Sichuan Province |
| 投稿时间：2018-04-08 修订日期：2018-05-15 |
| DOI：10.16688/j.zwbh.2018156 |
| 中文关键词: 山药黑斑病 病原鉴定 附球菌属 rpb2序列分析 |
| 英文关键词:black spot of yam pathogenic identification Epicoccum rpb2 sequence analysis |
| 基金项目:四川省科技计划项目（2016NYZ0053） |
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| 中文摘要: |
| 为鉴定引起山药茎蔓黑斑病的病原菌, 本研究利用柯赫氏法则验证其致病性, 根据菌株的形态特征和基于rpb2基因序列分析进行分类鉴定, 并初步测定其寄主范围。结果显示:分离的2株病原菌分生孢子椭圆形, 单胞, (3.9~5.1)μm×(1.8~2.7) μm, 产孢细胞瓶梗形, 分生孢子器近圆形或梨形, 无刚毛, 具孔口, 有时孔口延长形成乳突, NaOH颜色反应呈阳性, 参考Phoma鉴定手册, 可将其划分到section Peyronellaea。将供试菌的rpb2序列进行BLAST比对分析, 其与GenBank中的Epicoccum latusicollum具有最高的同源性, 支持率为97%, 结合形态学特征可将其鉴定为E. latusicollum, 该菌经人工接种后也能侵染番茄、辣椒和花菜幼苗。 |
| 英文摘要: |
| To identify the pathogen causing black rot of yam, experiments were performed, including pathogenicity test, host range determination, identification based on morphology and rpb2 sequences. The results showed that conidia of the pathogens were ellipsoidal, aseptate, (3.9-5.1) μm ×(1.8-2.7) μm, with phialidic conidiogenous cells, globose, pyriform and glabrous pycnidia, and sometimes elongated ostioles like a short, slightly papillate neck. The pathogens showed positive reactions in the NaOH test. Based on the morphology, the fungus was classified in section Peyronellaea according to the Phoma identification manual. The analysis of rpb2 sequences showed that the fungus shared the highest homology with Epicoccum latusicollum (up to 97%) in GenBank. Therefore, the fungus was identified as E. latusicollum, which could infect tomato, pepper and cauliflower in the seedling stage after inoculation. |
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