| 于 畅, 吴林娜, 赵立萍, 张晓晓, 杨玉文, 关 巍, 赵廷昌*.西瓜噬酸菌Ⅲ型分泌系统hrcQ基因功能分析[J].植物保护,2019,45(2):48-56. |
| 西瓜噬酸菌Ⅲ型分泌系统hrcQ基因功能分析 |
| Functional analysis of hrcQ in type Ⅲ secretion system of Acidovorax citrulli |
| 投稿时间：2018-04-27 修订日期：2018-06-29 |
| DOI：10.16688/j.zwbh.2018183 |
| 中文关键词: 西瓜噬酸菌 Ⅲ型分泌系统 hrcQ 基因功能分析 |
| 英文关键词:Acidovorax citrulli type Ⅲ secretion system hrcQ gene functional analysis |
| 基金项目:国家自然科学基金（31701754）；北京市自然科学基金（6162023）；国家西甜瓜产业技术体系（CARS-26）；国家重点研发计划（2016YFD0201004） |
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| 中文摘要: |
| Ⅲ型分泌系统(type Ⅲ secretion system, T3SS)是影响西瓜噬酸菌Acidovorax citrulli致病性的重要因素, hrcQ作为T3SS的保守基因, 在组成和维持该系统功能方面扮演着重要角色, 但hrcQ在西瓜噬酸菌中的具体生物学功能尚不明确。本试验以西瓜噬酸菌Aac5菌株为研究对象, 通过基因敲除、致病力及相关表型测定等, 解析了hrcQ基因的功能。结果表明:hrcQ缺失导致Aac5菌株对寄主的致病能力丧失, 运动性及生物膜形成能力降低, 并丧失了引起烟草过敏性反应能力; hrcJ和hrcR基因在hrcQ缺失突变株中的表达量下调, hrcQ对hrcJ和hrcR基因均为正调控。表明hrcQ在维持西瓜噬酸菌致病能力中起着不可或缺的作用。 |
| 英文摘要: |
| Acidovorax citrulli is the causal agent of bacterial fruit blotch, which causes serious losses in watermelon and melon industry. Type Ⅲ secretion system (T3SS) plays an important role in the pathogenicity of A. citrulli. The hrcQ is an important and conserved gene in forming and maintaining the function of T3SS. However, the biological function of this gene in A. citrulli is unclear. Therefore, in this study, we analyzed the functions of hrcQ by gene knockout, virulence and phenotypic determinations based on the wild type strain Aac5. The results showed that the hrcQ gene deletion mutant strain caused significant reduction in pathogenicity, swimming motility and biofilm formation, and it lost the ability to induce hypersensitive reaction in non-host Nicotiana tabacum. Besides, the expressions of hrcJ and hrcR genes were decreased in the mutant strain, indicating a positive regulation of hrcQ to both genes. More importantly, we confirmed that the deletion of hrcQ resulted in the loss of virulence of Aac5 on its host. Taken together, these results indicated that hrcQ was the key gene for maintaining the pathogenicity of A. citrulli. |
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