| 张 磊1#, 靳明辉1#, 张丹丹2, 姜玉英3, 刘 杰3, 吴孔明2, 萧玉涛1*.入侵云南草地贪夜蛾的分子鉴定[J].植物保护,2019,45(2):19-24. |
| 入侵云南草地贪夜蛾的分子鉴定 |
| Molecular identification of invasive fall armyworm Spodoptera frugiperda in Yunnan Province |
| 投稿时间：2019-03-13 修订日期：2019-03-14 |
| DOI：10.16688/j.zwbh.2019121 |
| 中文关键词: 草地贪夜蛾 DNA条码 单倍型 亚群 |
| 英文关键词:Spodoptera frugiperda DNA barcode haplotype subpopulation |
| 基金项目:转基因生物新品种培育重大专项(2016ZX08012-004)；国家自然科学基金(31601646) |
| 作者 | 单位 | | 张 磊1#, 靳明辉1#, 张丹丹2, 姜玉英3, 刘 杰3, 吴孔明2, 萧玉涛1* | 1. 中国农业科学院深圳农业基因组研究所, 深圳 518120
2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
3. 全国农业技术推广服务中心, 北京 100125 |
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| 中文摘要: |
| 草地贪夜蛾Spodoptera frugiperda(J.E. Smith)是一种原产于美洲热带和亚热带地区的重要农业入侵害虫, 目前已从缅甸进入我国云南西南部地区, 且呈现蔓延之势。对入侵物种快速准确鉴定是早期预警和监测预防的关键。本研究采用分子标记手段, 对采自云南5个县(市)的83份样品进行鉴定, 通过PCR扩增细胞色素C氧化酶第Ⅰ亚基(COⅠ)和磷酸甘油醛异构酶(Tpi)两个基因片段并进行序列测定分析。结果表明, 基于COⅠ基因片段的序列比对能够对草地贪夜蛾进行准确的物种鉴定, 并且能够对其近源种甜菜夜蛾等进行鉴定。基于Tpi基因序列的特异单倍型位点比较分析, 确认入侵我国的草地贪夜蛾均为“玉米型”。该研究为草地贪夜蛾的快速识别提供了方法支持, 同时为进一步的虫源地解析和遗传溯源积累了宝贵的前期数据。 |
| 英文摘要: |
| The fall armyworm, Spodoptera frugiperda (J.E. Smith), is an important agricultural invasive pest that is native to tropical and subtropical America. At present, the insect has crossed Myanmar and entered into southwestern Yunnan of China, and has the trend to overspread in the surrounding areaS. Rapid and accurate identification is the key to early warning, monitoring and prevention of this invasive specieS. In this study, genetic methods based on two molecular markers, mitochondrial cytochrome oxidase subunit Ⅰ gene (COⅠ) and triose-phosphate isomerase (Tpi), were used to characterize 83 samples collected from five different regions in Yunnan Province. The samples could be accurately identified to be S. frugiperda or close-related species such as S. exigua by sequence alignment based on COⅠ gene fragment. Subpopulation of “corn strain” was confirmed by Tpi haplotype analysiS. This study provides methodological support for rapid species identification of S. frugiperda, and provides early basic data for further origin analysis and genetic traceability. |
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