刘 娟, 冯 浩, 王 帅, 高小宁, 黄丽丽*.苹果树腐烂病菌对苯醚甲环唑的敏感性[J].植物保护,2019,45(1):170-173. |
苹果树腐烂病菌对苯醚甲环唑的敏感性 |
Sensitivity of Valsa mali to difenoconazole in China |
投稿时间:2018-03-19 修订日期:2018-05-10 |
DOI:S 436.611.11 |
中文关键词: 苹果黑腐皮壳菌 菌落直径 苯醚甲环唑 EC50 抗药性 |
英文关键词:Valsa mali colony diameter difenoconazole EC50 fungicide resistance |
基金项目:国家重点研发计划(2016YFD0201100) |
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中文摘要: |
为了明确苹果树腐烂病菌对苯醚甲环唑的敏感性现状, 本研究采用菌丝生长速率法测定了来自7个省份7个不同年份的106株苹果树腐烂病菌对苯醚甲环唑的敏感性, 并建立了敏感基线。结果表明:供试苹果树腐烂病菌菌株的EC50区间在0.003~0.123 μg/mL, 敏感性频率分布呈连续性单峰曲线。经K-S法检验符合正态分布, 未出现敏感性明显下降的抗性群体, 平均EC50 (0.044±0.029) μg/mL可作为苹果树腐烂病菌对苯醚甲环唑的敏感基线。不同地理来源的菌株对苯醚甲环唑的敏感性有显著性差异, 来自辽宁省的菌株敏感性最高, 河南省的敏感性最低; 不同年份的菌株敏感性也有显著性差异, 2009年采集的菌株对苯醚甲环唑的敏感性最高, 2016年采集的菌株敏感性最低, 菌株对药剂的敏感性随年份的推移逐渐降低, 说明病菌的抗药性不断增强。 |
英文摘要: |
To confirm the sensitivity of Valsa mali to difenoconazole, the sensitivity baseline was established by measuring the mycelial growth of 106 isolates collected from seven provinces in seven different years on the fungicide-amended media. The results indicated that 50% effective concentration (EC50) values ranged from 0.003 μg/mL to 0.123 μg/mL. Sensitivity frequency distribution showed a continuous single peak curve, which is a normal distribution based on analyzed results by K-S method. No resistant strain to difenoconazole was found in the tested isolates, and the average EC50 value (0.044 μg/mL±0.029 μg/mL) could be the sensitivity baseline of V.mali to difenoconazole. The isolates collected from different regions showed different sensitivities. The sensitivity of V.mali from Liaoning Province was the highest, while that from Henan Province was the lowest. Meanwhile, the sensitivity of V.mali collected in different years also showed significant difference to difenoconazole. The sensitivity of V.mali collected in 2009 was the highest, while that collected in 2016 was the lowest. The sensitivity of strains decreased along with years, indicating the resistance of V.mali to difenoconazole was increasing. |
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