| 李国平, 刘 冰, 黄建荣, 邱 峰, 封洪强*.转聚合cry1A.105、cry2Ab2和cp4epsps基因抗虫耐除草剂玉米的田间抗性评价[J].植物保护,2019,45(1):142-147. |
| 转聚合cry1A.105、cry2Ab2和cp4epsps基因抗虫耐除草剂玉米的田间抗性评价 |
| Field evaluation of Bt corn expressing cry1A.105+cry2Ab2+cp4epspsagainst Lepidoptera pests and tolerance to glyphosate |
| 投稿时间：2018-04-05 修订日期：2018-05-29 |
| DOI：S 435.13, S 513 |
| 中文关键词: 亚洲玉米螟 黏虫 棉铃虫 转基因玉米 抗性 |
| 英文关键词:Ostrinia furnacalis Mythimna separata Helicoverpa armigera transgenic Bt corn resistance |
| 基金项目:转基因重大专项(2016ZX08012004-007); 河南省科技开放合作基金(172106000054); 国家重点研发计划(2016YFD0300705) |
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| 中文摘要: |
| 为明确转聚合cry1A.105、cry2Ab2和cp4epsps基因的抗虫耐除草剂玉米的抗虫性和对除草剂的耐受性, 本文通过田间人工接虫, 以食叶级别、存活幼虫数、蛀孔数、隧道长度和雌穗被害级别等分别作为评价玉米心叶期和穗期的抗虫性标准, 评价了转聚合cry1A.105、cry2Ab2和cp4epsps基因，具有抗虫耐除草剂复合性状的玉米品种‘ND6708YGRR’和‘ND6515YGRR’及同型非转基因‘ND6708’、‘ND6515’和当地‘郑单958’品种对亚洲玉米螟、黏虫和棉铃虫的抗虫效果; 通过喷施除草剂后调查了玉米植株的存活率, 评价上述品种对除草剂的耐受性。田间试验结果表明:‘ND6708YGRR’和‘ND6515YGRR’两个转基因玉米品种对亚洲玉米螟、黏虫及棉铃虫的抗性水平均为高抗, 且在4叶期和8叶期喷施草甘膦后其受害率均为0, 而同型对照品种和当地对照品种喷施同剂量草甘膦后受害率为100%。综上, 聚合cry1A.105、cry2Ab2和cp4epsps基因，具有抗虫耐除草剂复合性状的玉米品种‘ND6708YGRR’和‘ND6515YGRR’对亚洲玉米螟、黏虫和棉铃虫具有较强的抗虫效果, 对草甘膦具有较好的耐受性。此研究可为将来建立以转Bt基因玉米为主要手段的综合防治技术体系提供依据。 |
| 英文摘要: |
| To quantify resistance against insect pests and tolerance to glyphosate of transgenic corn lines expressing cry1A.105+cry2Ab2+cp4epsps, the leaf feeding rating, larval survival rate, the number of borer holes and the tunnel length of corn lines/hybrids ‘ND6708YGRR’ and ‘ND6515YGRR’ containing its conventional non-transgenic cultivars ‘ND6708’ and ‘ND6515’, and ‘Zhengdan 958’, were evaluated by artificial infestations of the larvae of Ostrinia furnacalis (Guenée), Mythimna separata (Walker) and Helicoverpa armigera (Hübner) in field trials, respectively. Also, the mortality of plants after spraying herbicides was investigated to evaluate their tolerance to glyphosate. The results showed that corn lines/hybrids ‘ND6708YGRR’ and ‘ND6515YGRR’ expressing cry1A.105+cry2Ab2+cp4epsps were highly resistant to O. furnacalis, M. separata and H. armigera, and also performed excellent tolerance to glyphosate after spraying glyphosate with recommended dose at the 4 and 8 leaves stage, respectively. In contrast, the mortalities of conventional non-transgenic cultivars were 100% after spraying the same dose of glyphosate. These results demonstrate that transgenic corn lines expressing cry1A.105+cry2Ab2+cp4epsps were resistant to O. furnacalis, M. separata and H. armigera, and tolerant to glyphosate. This study will help to construct the strategy of planting transgenic Bt corn for controlling the Lepidopteran pests as one of main tactics in the future. |
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