| 何朋杰1#, 崔文艳1#, 何鹏飞1, 2, 袁 海1, 2, 李兴玉1, 2, 吴毅歆1, 2, 何月秋1, 2*.叶面喷施枯草芽胞杆菌XF-1防治大白菜根肿病[J].植物保护,2019,45(1):104-108. |
| 叶面喷施枯草芽胞杆菌XF-1防治大白菜根肿病 |
| Control of cabbage clubroot by foliar-spaying with Bacillus subtilis XF-1 |
| 投稿时间：2018-03-07 修订日期：2018-04-03 |
| DOI：S 476 |
| 中文关键词: 根肿病 枯草芽胞杆菌XF-1 氰霜唑 病害管理 土传病害 生物防治 |
| 英文关键词:clubroot disease Bacillus subtilis XF-1 cyazofamid disease management soil-borne disease biocontrol |
| 基金项目:国家自然科学基金(31560503); 云南农业大学自然科学青年基金(2016ZR02); 国家公益性行业(农业)科研专项(201003029) |
| 作者 | 单位 | | 何朋杰1#, 崔文艳1#, 何鹏飞1, 2, 袁 海1, 2, 李兴玉1, 2, 吴毅歆1, 2, 何月秋1, 2* | 1. 云南农业大学, 昆明 650201
2. 微生物菌种筛选与应用国家地方联合工程研究中心, 昆明 650217 |
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| 中文摘要: |
| 为了建立简易、有效的根肿病生防体系, 本研究采用浇灌和喷施枯草芽胞杆菌XF-1的绿色荧光蛋白标记菌株XF-1-gfp, 测定其在大白菜植株内的定殖能力, 并用野生型菌株XF-1发酵液研究其防治根肿病和挽回产量效果。结果表明, 叶面喷施XF-1-gfp发酵液后, 标记菌在大白菜根、茎、叶等组织内的密度呈现出“先上升后下降最后趋于平稳”的趋势, 最终稳定在约103 cfu/g组织; 盆栽试验结果表明, 与空白对照相比, 所有处理发病率和病情指数均显著下降, 喷施XF-1发酵液后最佳防治效果为56.4%, 浇灌化学杀菌剂10%氰霜唑悬浮剂2 000倍液及1×107 cfu/mL的XF-1发酵液防效分别为78.6%与70.7%。大田试验结果表明, 喷施XF-1发酵液后防治效果达 52.6%, 与浇灌化学杀菌剂10%氰霜唑悬浮剂(64.6%)及XF-1发酵液(74.0%)相比无显著差异。此外, 与空白对照、浇灌10%氰霜唑悬浮剂及XF-1发酵液相比, 喷施XF-1发酵液后大白菜单株产量分别增加了74.0%、35.1%及23.6%。试验结果表明叶面喷施XF-1发酵液是一种有效的防控大白菜根肿病及增产增收的方法。 |
| 英文摘要: |
| To establish an effective and simple biocontrol management system in the field, the colonization ability of XF-1 was tested in Chinese cabbage by drenching and spraying XF-1-gfp and clubroot control and yield recovery effects were studied by drenching and spraying the wild strain XF-1 in the pot and field experiments. The results showed that its population in root, stem and leaf of cabbage after foliar-spraying had a rising trend first, then decreasing and leveling off, and finally the colonization density kept at 103 cfu/g tissues. The incidence rate and disease index had a significant decline compared to the negative control in the pot experiment with 56.4% of control effect by spraying XF-1, 78.6% and 70.7% by drenching 2 000 times dilution of cyazofamid and 1×107cfu/mL of XF-1. The disease control effects were 52.6%, 64.6% and 74.0%, respectively, in the field experiment without significant difference. Moreover, spraying XF-1 increased yield by 70.4%, 35.1% and 23.6% compared with the negative control, drenching cyazofamid and XF-1, respectively. This study suggested that foliar-spray of XF-1 might be an effective way for controlling clubroot and increasing Chinese cabbage yield. |
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