| 张梦园, 潘 锐, 谭乐勇, 郭 敏*.稻瘟病菌F-box蛋白MoFbr7生物学功能分析[J].植物保护,2019,45(1):19-24. |
| 稻瘟病菌F-box蛋白MoFbr7生物学功能分析 |
| Functional analysis of F-box protein MoFbr7 in Magnaporthe oryzae |
| 投稿时间：2018-03-28 修订日期：2018-05-21 |
| DOI：S 435.111.41 |
| 中文关键词: 稻瘟病菌 F-box蛋白 基因敲除 功能分析 |
| 英文关键词:Magnaporthe oryzae F-box protein gene knock-out functional analysis |
| 基金项目:安徽省自然科学基金青年基金(1608085QC49) |
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| 中文摘要: |
| F-box蛋白在泛素-蛋白酶体途径(ubiquitin-proteasome pathway, UPP)中参与调控胞内蛋白降解、受体识别、信号传导等生物学过程。本研究从稻瘟病菌中克隆了F-box基因MoFbr7, 序列分析表明, 该基因编码产物具有1个F-box结构域(N-端)和8个连续的WD40重复序列(C-端), 在丝状真菌中高度保守。利用基因敲除方法, 获得4个MoFbr7基因敲除突变体, 同时构建了回补菌株。表型分析结果显示, MoFbr7基因缺失突变体在产孢量、附着胞形态、原生质体释放、致病性等方面均无异常。突变体在MM、RDC培养基上生长速率下降; 对细胞壁胁迫因子CFW(Calcofluor white)、刚果红敏感。以上结果表明MoFbr7参与稻瘟病菌的营养生长与细胞壁完整性, 为进一步揭示其生物学功能奠定基础。 |
| 英文摘要: |
| F-box protein is involved in the regulation of intracellular protein degradation, receptor recognition, signal transduction and other biological processes in the ubiquitin-proteasome pathway (UPP). In this study, we identified an F-box protein MoFbr7 in the rice blast fungus. Sequence analysis showed that MoFbr7 had an F-box domain at the N-terminal and a WD40 repeats at the C-terminal, and was highly conserved among filamentous fungi based on the phylogenetic analysis. Four knockout mutants of MoFbr7 were obtained by gene knockout method and a complementary strain was constructed based on the deletion mutant. Phenotypic analysis showed that there were no significant differences in sporulation, conidial production, protoplast release and pathogenicity compared with the wild-type stain Guy11. However, the growth rate of ΔMoFbr7 was significantly decreased on MM and RDC media. The knockout strains were sensitive to the cell wall stressors Calcofluor white (CFW) and Congo Red (CR). The above results indicated that MoFbr7 was involved in vegetative growth and cell wall integrity, supplying the foundation for further revealing its biological functions. |
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