| 朱海霞 #, 马永强 #, 郭青云 *.极细链格孢菌剂的初步研制及其除草作用研究[J].植物保护,2018,44(5):212-216. |
| 极细链格孢菌剂的初步研制及其除草作用研究 |
| Development of the biocontrol agents of Alternaria tenuissima HZ-1 and its herbicidal activity |
| 投稿时间：2018-04-25 修订日期：2018-06-07 |
| DOI：10.16688/j.zwbh.2018177 |
| 中文关键词: 极细链格孢菌HZ-1 菌剂研制 除草效果 |
| 英文关键词:Alternaria tenuissima HZ-1 development of agent control effect to weeds |
| 基金项目:国家自然科学基金 (31560518); 中国科学院“西部之光”人才培养引进计划 (201523) |
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| 中文摘要: |
| 本研究选用一株具有除草活性的生防菌株极细链格孢Alternaria tenuissima HZ-1作为研究对象, 通过载体和助剂的筛选, 初步研制出可湿性粉剂, 并对杂草离体叶片、盆栽杂草及田间小区杂草的致病效果进行了测定。结果表明, HZ-1最适载体为高岭土，助剂为羧甲基纤维素、可溶性淀粉和白炭黑, 菌株HZ-1可湿性粉剂对不同杂草离体叶片致病性表现为: 密花香薷＞冬葵＞野燕麦＞薄蒴草＞酸模叶蓼＞藜＞萹蓄。盆栽致病性结果显示, HZ-1可湿性粉剂对薄蒴草、藜和密花香薷致病效果突出, 对冬葵和酸模叶蓼次之, 对萹蓄、野燕麦无效。小区试验显示对繁缕、藜和密花香薷有显著除草效果。该菌株可作为防除薄蒴草、藜、密花香薷和繁缕等杂草的生物除草剂进行进一步研究。 |
| 英文摘要: |
| Alternaria tenuissima HZ-1 was selected as a high virulent strain to weeds. Proper carrier and auxiliary were screened and wettable powder was developed as biological herbicide, and the virulence to different weeds in vitro and in vivo was also determined. The results showed that the optimal vector of HZ-1 was kaolin, the auxiliarges were carboxymethyl cellulose, soluble starch and silica. The susceptibility of strain HZ-1 wet powder to different weeds in vitro was as follows: Elsholtzia densa Benth.＞Malva verticillata L.var. crispa＞Avena fatua L.＞Lepyrodiclis holosteoides (C.A.Mey.) Fisch.et Mey.＞Polygonum lapathifolium L.＞Chenopodium album L.＞Polygonum aviculare L.. Pot experiments showed that wet powder of HZ-1 had significant virulence to L. holosteoides, C. album and E. densa, but no virulence to P. aviculare and A. fatua. Field trials demonstrated that wet powder of HZ-1 was highly virulent to Stellaria media, C. album and E. densa. This strain could be further studied as a biological herbicide to control L. holosteoides, C. album, E. densa and Stellaria media. |
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