| 战斌慧, 周雪平*.高通量测序技术在植物及昆虫病毒检测中的应用[J].植物保护,2018,44(5):120-126. |
| 高通量测序技术在植物及昆虫病毒检测中的应用 |
| Application of next-generation sequencing technology indetection of plant and insect viruses |
| 投稿时间：2018-07-23 |
| DOI：10.16688/j.zwbh.2018326 |
| 中文关键词: 第二代测序 植物病毒 昆虫病毒 检测 |
| 英文关键词:next-generation sequencing plant virus insect virus detection |
| 基金项目:国家自然科学基金重大项目 (31390422) |
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| 摘要点击次数: 1076 |
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| 中文摘要: |
| 在过去的十几年中, 测序技术的发展为分子生物学领域带来了革命性的变化。第二代测序 (next-generation sequencing, NGS)技术以快速、高灵敏性、高通量、非序列依赖性等特点极大地促进了病毒诊断学研究领域的发展。NGS技术可以在不了解病毒的生物学特性、血清学特点及基因组信息情况下快速检测未知病毒。通过对总核酸样本进行NGS可以获得某个特定生态环境或种植系统中的所有病毒序列, 即病毒组。通过大量的NGS数据可以分析寄主中某一病毒的基因组变化、构建病毒的准种以及研究病毒的进化和起源。本文介绍了高通量测序的方法在植物和昆虫病毒检测中的应用。 |
| 英文摘要: |
| In the past decade, the development of sequencing technology has induced revolutionary changes in molecular biology. Next-generation sequencing (NGS) has considerably promoted the development of research on viral diagnostics characterized by rapidity, high sensitivity, high throughput, sequence-independent, etc. NGS made it possible to rapidly detect unknown viruses without knowing the biological characteristics, serological characteristics, and genome information. The whole viral genomes in a particular ecosystem or cropping system, namely virome, could be sequenced through NGS of the total nucleic acids. Viral genome variability, reconstruction of quasispecies, evolution and origin within the host can be obtained by analyzing massive NGS data. In this article, we provide the overview of the process of NGS technology and its applications in detection of plant and insect viruses. |
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