| 严婉荣, 陈 圆, 肖 敏, 赵志祥*, 肖彤斌*.海南玉米小斑病菌室内药剂筛选及对戊唑醇、嘧菌酯的敏感性[J].植物保护,2018,44(4):183-187. |
| 海南玉米小斑病菌室内药剂筛选及对戊唑醇、嘧菌酯的敏感性 |
| Screening of fungicides and sensitivity of Bipolaris maydis totebuconazole and azoxystrobin in Hainan Province |
| 投稿时间：2017-09-19 修订日期：2017-11-27 |
| DOI：10.16688/j.zwbh.2017366 |
| 中文关键词: 玉米小斑病菌 戊唑醇 嘧菌酯 敏感性 |
| 英文关键词:Bipolaris maydis tebuconazole azoxystrobin sensitivity |
| 基金项目:国家公益性行业(农业)科研专项(201403075); 省属科研院所技术开发研究专项(KYYS201501) |
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| 中文摘要: |
| 为明确7种杀菌剂对玉米小斑病菌CM-4的抑菌效果和玉米小斑病菌对戊唑醇和嘧菌酯的敏感性, 采用菌丝生长速率法测定了7种杀菌剂对菌株CM-4的抑菌效果, 并根据初筛结果, 对来自海南省8个市县的36株玉米小斑病菌进行戊唑醇和嘧菌酯的敏感性测定。结果显示:7种药剂中, 430 g/L戊唑醇SC和250 g/L嘧菌酯SC的抑菌效果较好, 70%甲基硫菌灵WP和50%多灵菌WP的抑菌效果较差。敏感性测定中, 430 g/L戊唑醇SC的EC50均值为7.389 2 μg/mL, 敏感性频率分布出现了2个明显的峰值, 检验结果不符合正态分布, 出现敏感性下降的菌株。250 g/L嘧菌酯SC的敏感性频率分布呈现单一连续性曲线, 检验结果符合正态分布, 其EC50均值5.699 5 μg/mL可作为玉米小斑病菌对嘧菌酯的敏感性基线。敏感性基线的建立可为病原菌抗性研究和监测提供基础数据, 同时指导田间用药。 |
| 英文摘要: |
| To clear the effect of 7 fungicides to Bipolaris maydis CM-4 and the sensitivities of B.maydis to tebuconazole and azoxystrobin, toxicity of 7 fungicides to CM-4 were determined according to the rates of the mycelial growth of B. maydis in media containing the fungicides, the sensitivities of B.maydis to tebuconazole and azoxystrobin were determined using 36 isolates collected from 8 counties of Hainan Province. The results showed that the inhibitory efficacy of tebuconazole 430 g/L SC and azoxystrobin 250 g/L SC to CM-4 was better, while that of thiophanate 70% WP and carbendazim 5% WP was lower. In the sensitivity test, the EC50 value of tebuconazole 430 g/L SC was 7.389 2 μg/mL, and the sensitivity frequency distribution showed two obvious peaks. Further analysis revealed that there exist low resistant sub-colony to tebuconazole. The sensitivity frequency distribution of isolates to azoxystrobin 250 g/L SC showed a single continuous curve and followed normal distribution. The EC50 value of azoxystrobin was 5.699 5 μg/mL, which could be used as a sensitive baseline for azoxystrobin. The establishment of a sensitivity baseline provides a data basis for the study and monitoring of pathogen resistance, meanwhile guiding the fungicide application in practice. |
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