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齐恩芳 1, 2, 3 , 刘 石 1, 2, 3, 贾小霞 1, 2, 3 , 文国宏 1, 2, 3, 吕和平 1, 2, 3, 黄 伟 1, 2, 高彦萍 1.甘肃省马铃薯主要病毒病发生情况调查[J].植物保护,2018,44(4):171-176.
甘肃省马铃薯主要病毒病发生情况调查
Investigation and analysis of important potato viral diseases in Gansu Province
投稿时间:2017-09-04  修订日期:2017-10-12
DOI:10.16688/j.zwbh.2017341
中文关键词:  马铃薯  病毒病  调查鉴定
英文关键词:potato  virus disease  investigation
基金项目:国家自然科学基金(31360353); 甘肃省农业科学院农业科技创新专项计划(2017GAAS39); 甘肃省农业生物技术研究与应用开发项目(GNSW-2014-13, GNSW-2016-15); 国家重点研发计划(2017YFD0201600, 2017YFD0201602-4)
作者单位
齐恩芳 1, 2, 3 , 刘 石 1, 2, 3, 贾小霞 1, 2, 3 , 文国宏 1, 2, 3, 吕和平 1, 2, 3, 黄 伟 1, 2, 高彦萍 1 1.甘肃省农业科学院马铃薯研究所, 兰州 730070
2. 甘肃省马铃薯种质资源创新工程实验室, 兰州 730070
3. 农业部西北旱作马铃薯科学观测实验站, 甘肃 748201 
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中文摘要:
      2015年-2016年, 在甘肃省10个地市24个马铃薯主栽县(区)146个生态区域(乡镇)采集了757份具有典型症状的马铃薯样品, 应用DAS-ELISA法进行检测, 筛查6种主要病毒(PVX、PVY、PLRV、PVA、PVS和PVM)。结果表明:631份样品检测到病毒, PVS的检出率最高, 达47.03%, PVY次之, 为33.82%, PVA最低, 只有0.63%; 发生复合侵染的病毒主要为PVY+PVS, 复合侵染率达到10.13%, 三种病毒复合侵染主要是PVY+PVS+PVM; 病毒种类和感病程度与品种、地域有关。
英文摘要:
      757 potato samples with virus-like symptoms were collected from 146 different ecological regions (villages and towns) in 24 potato main planting counties and districts which are affiliated to 10 cities and prefectures in Gansu Province. All samples were tested for the presence of six viruses (PVX, PVY, PLRV, PVA, PVS and PVM) by DAS-ELISA in this study. A total of 631 samples were shown to be infected with virus, of which 47.03% were infected with PVS, and 33.82% with PVY. Two or three viruses were detected from some potato plants, and the combination infection of PVY and PVS was the main type with the highest infection rate of 10.13%, and the mixed-infection of PVY, PVS and PVM was the main type of infection containing three kind of virus. The type of virus and disease susceptibility were related to the potato varieties and potato growing regions.
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