| 徐 爽, 陈立杰, 王媛媛, 朱晓峰, 刘晓宇, 范海燕, 段玉玺*.诱导小麦抗叶锈病生防细菌的筛选、鉴定及其防效评价[J].植物保护,2018,44(4):47-52. |
| 诱导小麦抗叶锈病生防细菌的筛选、鉴定及其防效评价 |
| Identification of the biocontrol bacterial strains for induced resistance against wheat leaf rust and control effect evaluation |
| 投稿时间：2017-11-07 修订日期：2018-01-02 |
| DOI：10.16688/j.zwbh.2017427 |
| 中文关键词: 小麦叶锈病 多黏类芽胞杆菌 诱导抗性 防效 |
| 英文关键词:wheat leaf rust Paenibacillus polymyxa induced resistance control efficacy |
| 基金项目:国家现代农业产业技术体系岗位科学家专项(CARS-04-PS13) |
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| 中文摘要: |
| 小麦叶锈病是小麦生产上的重要病害, 防治该病害主要采用抗病品种和喷施化学农药, 而探索生物防治是防治该病的一种新方法。本研究使用3株细菌菌悬液对小麦种子进行处理, 于小麦一叶期接种叶锈病菌10 d后调查其发病率和严重度。结果表明, 在盆栽试验中, Sneb1462菌株菌悬液诱导小麦抗叶锈病的效果最好, 可使发病率和严重度比对照分别降低27.54%和49.90%; Sneb1462还可促进小麦根部生长, 施用后小麦根长和地下部鲜重分别比对照提高30.19%和29.03%。在大田试验中, 用Sneb1462菌悬液处理小麦种子后叶锈病的严重度降低38.60%, 小麦株高和穗重分别提高16.44%和34.98%, 表明该菌株是一株优良的抗病促生菌。经透射电镜观察、16S rDNA序列分析及生理生化的检测, 鉴定该菌株为多黏类芽胞杆菌Paenibacillus polymyxa。利用生防细菌Sneb1462进行种子处理防控小麦叶锈病将是一种新型的植保措施, 具有重要的研究意义。 |
| 英文摘要: |
| Wheat leaf rust is an important wheat disease, which can be controlled by using resistant varieties and chemical fungicides, and biological control is a new method. In this study, wheat seeds were coated with the fermentation broth of three biocontrol strains that were selected in a field experiment. The seedlings were inoculated by spraying spore suspension of Puccinia triticina. The incidence and severity were investigated 10 days after inoculation. The results showed that wheat seeds coated with strain Sneb1462 had obvious control efficacy against wheat leaf rust in pot experiments: the incidence and severity decreased by 27.54% and 49.90%, and the length and fresh weight of wheat root increased by 30.19% and 29.03%, respectively. The severity decreased by 38.60%, and the plant height and spike weight of wheat increased by 16.44% and 34.98% in the field, respectively. It was clear that this strain was a plant growth-promoting rhizobacteria, which was identified as Paenibacillus polymyxa based on morphological and biochemical characteristics as well as 16S rDNA sequence analysis. It may be a new type of plant protection measure for controlling wheat leaf rust by coating seeds with the strain Sneb1462, and therefore has important research significance. |
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