| 齐永霞, 陈方新.玉米根际球孢白僵菌群体遗传多样性的ISSR分析[J].植物保护,2018,44(3):67-73. |
| 玉米根际球孢白僵菌群体遗传多样性的ISSR分析 |
| Genetic diversity of Beauveria bassiana in maize rhizosphere by inter-simple sequence repeat (ISSR) markers |
| 投稿时间：2017-05-18 修订日期：2017-07-20 |
| DOI：10.16688/j.zwbh.2017184 |
| 中文关键词: 球孢白僵菌 分子标记 遗传分化 遗传多样性 |
| 英文关键词:Beauveria bassiana molecular marker genetic differentiation genetic diversity |
| 基金项目:安徽农业大学稳定和引进人才科研资助项目(2011); 安徽农业大学青年基金资助项目(2011-31) |
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| 中文摘要: |
| 为了明确玉米根际球孢白僵菌的遗传分化情况及亲缘关系, 通过ISSR-PCR分子标记技术对分离自玉米根际的球孢白僵菌的遗传多样性进行了研究。从40个引物中共筛选出11个多态性高、稳定性好的引物用于正式的扩增分析, 在37个菌株中共扩增出83条谱带, 其中多态性条带占69条, 多态性百分率为83.13%。平均每引物扩增条带在7.5条。群体的多态位点百分率(PPL)为83.13%, Nei基因多样性指数(H)为0.316 9, Shannon信息指数(I)为0.465 7。结果表明, 分离自安徽省涡阳、萧县、蒙城三个地区的球孢白僵菌具有较高的遗传多样性。研究结果对进一步探讨玉米根际球孢白僵菌不同菌株的生防效果具有重要意义。 |
| 英文摘要: |
| To identify the genetic differentiation and genetic relationships among the isolates of Beauveria bassiana from maize rhizosphere, the genetic diversity of 37 B. bassiana strains was estimated by using inter-simple sequence repeats (ISSR) markers. Eleven of 40 ISSR primers were chosen for analysis of their reproducibility and high polymorphism. Each primer resulted in 7.5 markers and totally 83 fragments were amplified, in which 69 (83.13%) were polymorphic. Genetic diversity analysis revealed a relatively high level of intraspecific genetic diversity of B. bassiana. The percentage of polymorphic loci (PPL) was 83.13%; Nei’s genetic diversity (H) was 0.316 9 and Shannon’s information index (I) was 0.465 7. It suggested that the 37 B. bassiana strains isolated from Guoyang, Xiaoxian and Mengcheng had high genetic diversity. The results were of great significance for further studying the biological control effect of B. bassiana strains. |
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