| 李沛利, 刘 丹, 陈诗瑶, 龚国淑*.四川省成都市狭叶十大功劳炭疽病病原菌的鉴定与生物学特性研究[J].植物保护,2018,44(3):61-66. |
| 四川省成都市狭叶十大功劳炭疽病病原菌的鉴定与生物学特性研究 |
| Identification and biological characteristics of the pathogen causing anthracnose on Mahonia fortunei in Chengdu, Sichuan |
| 投稿时间：2017-10-02 修订日期：2017-12-03 |
| DOI：10.16688/j.zwbh.2017381 |
| 中文关键词: 十大功劳 炭疽病 病原鉴定 生物学特性 |
| 英文关键词:Mahonia fortunei anthracnose pathogen identification biological characteristics |
| 基金项目:四川农业大学双支计划(03572101); 四川农业大学导师培育计划(04051835) |
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| 中文摘要: |
| 为弄清狭叶十大功劳炭疽病的病原菌, 从四川省成都市采集疑似为炭疽病的病叶, 采用组织分离法分离病原菌, 单孢纯化后通过柯赫氏法则验证为致病菌; 根据形态学特征, 结合多基因系统学, 将病原菌鉴定为果生炭疽菌 Colletotrichum fructicola。该菌对温度的适应范围较广, 10～35℃之间均能生长和产孢, 最适生长和产孢温度均为30℃; 光照对菌丝生长和产孢影响不显著; 在pH 3～11之间均能生长和产孢, 菌丝生长最适pH为7, 产孢最适pH为4; 葡萄糖、甘露醇、乳糖和麦芽糖是菌丝生长的最适碳源, 麦芽糖为产孢的最适碳源; 蛋白胨是生长和产孢的最适氮源。 |
| 英文摘要: |
| In order to identify the pathogen causing anthracnose of Mahonia fortunei, small pieces of tissues were taken from the leaves which exhibited symptoms of anthracnose, and through tissue separation, single-spore purification and Koch’s postulate, the pathogenic isolate was confirmed. Based on morphological characteristics and multi-locus sequences, the isolate was identified as Colletotrichum fructicola. The isolate had a wide range of temperature adaptation; the temperature range for mycelium growth and sporulation was from 10℃ to 35℃ and the optimum temperature was 30℃. The light had no remarkable effect on the mycelial growth and sporulation. The pH range for mycelium growth and sporulation was from 3 to 11, and the optimum pH for mycelium growth and sporulation was 7 and 4, respectively. The optimal carbon sources for mycelial growth were dextrose, mannitol, maltobiose and lactose, and for sporulation, maltobiose. The optimal nitrogen source for mycelial growth and sporulation was peptone. |
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