| 毛迎新1, 谭荣荣1, 王友平2, 陈 勋1, 王红娟1, 黄丹娟1, 龚自明1*.基于16S rDNA序列的小贯小绿叶蝉共生细菌多样性研究[J].植物保护,2018,44(3):17-23. |
| 基于16S rDNA序列的小贯小绿叶蝉共生细菌多样性研究 |
| Analysis of the bacterial diversity in adults of Empoasca (Matsumurasca) onukii based on 16S rDNA sequences |
| 投稿时间：2017-09-06 修订日期：2017-10-24 |
| DOI：10.16688/j.zwbh.2017347 |
| 中文关键词: 小贯小绿叶蝉 细菌 16S rDNA 多样性 |
| 英文关键词:Empoasca (Matsumurasca) onukii bacterium 16S rDNA diversity |
| 基金项目:国家重点研发计划(2016YFD0200905); 湖北省农业科技创新中心团队项目(2016-620-000-001-032); 湖北省第二批现代农业产业技术体系项目 |
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| 中文摘要: |
| 利用Illumina HiSeq技术, 对赤壁、大悟、武汉、咸安和英山5个茶园小贯小绿叶蝉地理种群的成虫共生细菌16S rDNA-V4变异区进行测序, 应用Uparse和RDP Classifier等软件统计和分析样本的物种组成、丰度和多样性。5个地区小贯小绿叶蝉成虫的16S rDNA基因序列文库共获得239 645条有效tags, 在97%相似阈值下将其聚类为3 403个OTUs。共注释到41个门, 116个纲, 197个目, 272个科, 372个属, 105个种。5个样本的共生菌在不同分类水平上的组成有所不同, 其中在门水平上, 主要优势菌为变形菌门Proteobacteria(相对丰度60.6%~97.1%); 在纲水平上, 相对丰度排名前5位中共有的优势菌为 γ-变形菌纲Gammaproteobacteria和α-变形菌纲Alphaproteobacteria; 在属水平上, 排名前10位中5个样本共有的优势菌为盐单胞菌属 Halomonas、希瓦氏菌属 Shewanella 和沃尔巴克氏体属 Wolbachia。小贯小绿叶蝉成虫细菌的Chao指数、Ace指数、Shannon指数和Simpson指数分别为1 065.55~2 841.89, 1 130.76~2 914.90, 1.07~8.63和0.18~0.99。茶园小贯小绿叶蝉成虫共生细菌多样性比较丰富, 不同地理种群的小贯小绿叶蝉细菌群落结构和多样性有差异。本研究结果为进一步研究细菌对小贯小绿叶蝉种群生物学的影响奠定了基础。 |
| 英文摘要: |
| The V4 regions of the 16S rDNA of symbiotic bacteria in adults of Empoasca (Matsumurasca) onukii from five geographical regions including Chibi, Dawu, Wuhan, Xian’an and Yingshan were sequenced by Illumina HiSeq techniques. The numbers of operational taxonomic units (OTUs), bacterial community, species abundance and alpha diversity in samples were analyzed by using Uparse and RDP Classifier software. The results showed that a total of 239 645 effective tags were obtained, which were clustered into 3 403 OTUs, and annotated into 41 phyla, 116 classes, 197 orders, 272 families, 372 genera, and 105 species. At the phyla level, Proteobacteria was the common dominant bacteria (relative abundance 60.6%-97.1%). Gammaproteobacteria and Alphaproteobacteria were the commonly dominant classes in the top 5 classes of bacterial communities between the five samples. Halomonas, Shewanella and Wolbachia were the commonly dominant genera in the top 10 genera of bacterial communities. The indexes of Chao, Ace, Shannon and Simpson were 1 065.55-2 841.89, 1 130.76-2 914.90, 1.07-8.63 and 0.18-0.99, respectively. The results indicated that there are diverse bacteria in adults of E.（M.）onukii, and there are differences in the bacterial community diversity among E. (M.) onukii populations. These results provide a basis for further study of the effects of bacteria on the biology of E. (M.) onukii. |
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