| 王思敏, 朱婕妮, 张迪, 康涛, 李佳玲, 梁俊玉*.铺散亚菊挥发油化学成分组成及其对马铃薯茎线虫的毒杀作用[J].植物保护,2018,44(2):154-157. |
| 铺散亚菊挥发油化学成分组成及其对马铃薯茎线虫的毒杀作用 |
| Chemical composition and contact toxicity of essential oils extracted from Ajania khartensis against Ditylenchus destructor |
| 投稿时间：2017-05-18 修订日期：2017-08-28 |
| DOI：10.16688/j.zwbh.2017185 |
| 中文关键词: 铺散亚菊 挥发油 马铃薯茎线虫 杀线虫活性 |
| 英文关键词:Ajania khartensis essential oil Ditylenchus destructor nematicidal activity |
| 基金项目:国家自然科学基金(81660632) |
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| 中文摘要: |
| 马铃薯茎线虫是造成当归麻口病的主要原因。本试验研究了铺散亚菊挥发油的化学组成及其对马铃薯茎线虫的毒杀作用。采用水蒸气蒸馏法提取挥发油, GC/MS分析了挥发油的成分及其相对含量; 贝曼漏斗法从当归中分离马铃薯茎线虫, 以灰葡萄孢培养线虫; 药液浸泡法测定挥发油的杀线虫活性。结果显示:从铺散亚菊挥发油中鉴定出25种化合物, 占挥发油总成分的98.44%, 其主要成分为樟脑(13.20%)、桉叶油醇 (13.16%)、百里香酚(10.96%)、β -水芹烯(9.66%)、伞柳酮(9.40%)、4-乙基-1-(1-甲基乙基)-二环［3.1.0］-2-戊烯(7.60%)和侧柏酮(6.18%)。铺散亚菊挥发油对马铃薯茎线虫的LC50为3.83 mg/mL, 而阳性对照35%甲基异柳磷乳油的LC50为0.14 mg/mL。铺散亚菊挥发油对当归茎线虫有较好的毒杀作用, 能够为铺散亚菊挥发油防治马铃薯茎线虫提供一定的理论依据, 对提高当归的质量和产量有一定意义。 |
| 英文摘要: |
| Ditylenchus destructor is one of the main causes for Makou disease of Angelica sinensis. Here the nematicidal activity of Ajania khartensis essential oils against D. destructor was investigated. The essential oils were extracted by steam distillation method, and the compositions of A. khartensis essential oils were analyzed by GC-MS. D. destructor was obtained by using the Berman funnel method from A. sinensis infected with Makou disease. The nematicidal activity of A. khartensis essential oils against D. destructor was tested by liquid immersion method. The results showed that 25 compounds were identified and the sum of their contents accounted for 98.44% of the essential oils. The main components of A. khartensis essential oils were camphor (13.20%), eucalyptol (13.16%), thymol (10.96%), β-phellandrene (9.66%), umbellulone (9.40%), 4-ethyl-1-1-methyl ethyl）-bicycto［3.1.0］-2-pentene (7.60%) and thujone (6.18%). The essential oils of A.khartensis showed certain nematicidal activity (LC50=3.83 mg/mL) compared with the positive control isofenphos-methyl 35% EC (LC50=0.14 mg/mL) against D. destructor. These results can provide the theoretical basis for controlling D. destructor by using A. khartensis essential oils. |
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