| 吕苗苗, 李文学, 孙牧笛, 胡丽杰, 顾沛雯*.葡萄卷叶伴随病毒1号和3号宁夏分离物部分基因序列分析[J].植物保护,2018,44(1):74-80. |
| 葡萄卷叶伴随病毒1号和3号宁夏分离物部分基因序列分析 |
| Sequence analysis of the genes of Grapevine leafroll-associated viruses 1 and 3 from Ningxia isolates |
| 投稿时间：2017-05-20 修订日期：2017-08-02 |
| DOI：10.16688/j.zwbh.2017187 |
| 中文关键词: 葡萄卷叶伴随病毒 (GLRaVs) RT-PCR检测 克隆 序列分析 |
| 英文关键词:Grapevine leafroll-associated virus (GLRaVs) RT-PCR detection cloning sequence analysis |
| 基金项目:宁夏教育厅项目 (NGY14011); 宁夏重点研发计划重大项目 (2016BZ06) |
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| 中文摘要: |
| 为明确葡萄卷叶伴随病毒 (GLRaVs) 在宁夏贺兰山东麓酿酒葡萄上的侵染状况, 采用RT-PCR技术对40份酿酒葡萄样品中的GLRaV-1~GLRaV-5进行了外壳蛋白 (CP) 、复制酶 (RdRp) 和热激蛋白 (HSP70) 基因序列的克隆和分析。检测结果表明, 在所检测的5种病毒中, 除GLRaV-2和GLRaV-4未检测到外, GLRaV-1和GLRaV-3的检出率最高, 分别为20.0%和32.5%, GLRaV-5的检出率仅为5.0%; 有6个样品存在GLRaV-1和GLRaV-3两种病毒复合侵染。序列分析表明, GLRaV-1宁夏分离物的部分CP基因序列长度为232 nt, 其两种分离物间的核苷酸序列同源率为90%, 与已报道的国内外其他分离物CP基因序列相比, 其同源率为90%~99%; GLRaV-3宁夏分离物的CP基因序列长度为942 nt, 其两种分离物间的核苷酸序列同源率为40%, 与已报道的国内外其他分离物CP基因序列相比, 其同源率为40%~99%; GLRaV-3宁夏分离物的RdRp基因序列长度为683 nt, 其各分离物间的核苷酸序列同源率为90%以上, 与已报道的国内外其他分离物RdRp基因序列相比, 其同源率为90%~99%; GLRaV-3宁夏分离物的HSP70基因序列长度为546 nt, 其两种分离物间的核苷酸序列同源率为96%, 与已报道的国内外其他分离物HSP70基因序列相比, 其同源率为96%~99%。 |
| 英文摘要: |
| To understand the infection status of Grapevine leaf roll-associated virus in wine grapes grown in Helan Mountain East Region of Ningxia, RT-PCR was used to clone the coat protein (CP), replication enzyme (RdRp) and heat shock protein (HSP70) gene sequences of Grapevine leaf roll-associated virus 1-5 in 40 wine grape samples and gene sequence analysis was conducted. The results showed that the detection rate of GLRaV-1 and GLRaV-3 in all samples was 20.0% and 32.5%, respectively, but the detection rate of GLRaV-5 was only 5.0%; in addition, GLRaV-2 and GLRaV-4 were not detected in the samples, GLRaV-1 and GLRaV-3 showed compound infection in six samples. The sequence analysis showed that each partial sequence of GLRaV-1-CP gene of the two isolates was 232 nt long and their nucleotide sequence homology was 90%, and their nucleotide sequence homology with those of previously reported isolates was 90%-99%. Each of the complete sequence of GLRaV-3-CP gene of the two isolates was 942 nt long, sharing a nucleotide sequence homology of 40%. Their nucleotide sequence homology with those of previously reported isolates was 40%-99%. Each of the complete sequence of GLRaV-3-RdRp gene of the three isolates was 683 nt long, sharing a nucleotide sequence homology of 90%, and their nucleotide sequence homology with those of previously reported isolates was 90%-99%. Each of the complete sequence of GLRaV-3-HSP70 gene of the two isolates was 546 nt long, sharing a nucleotide sequence homology of 96%, and their nucleotide sequence homology with those of previously reported isolates was 96%-99%. |
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