| 严丹侃 1, 郑红英 2, 张海珊 1, 沈 艳 1, 顾江涛 1, 章东方 1*, 燕 飞 2*.蚕豆萎蔫病毒2号安徽分离物全基因组序列测定与分析[J].植物保护,2018,44(1):67-73. |
| 蚕豆萎蔫病毒2号安徽分离物全基因组序列测定与分析 |
| Identification and analysis of complete genomic sequence of Broad bean wilt virus 2 Anhui isolate |
| 投稿时间：2017-05-02 修订日期：2017-06-12 |
| DOI：10.16688/j.zwbh.2017158 |
| 中文关键词: 蚕豆萎蔫病毒2号 安徽分离物 基因组 序列分析 |
| 英文关键词:Broad bean wilt virus 2 Anhui isolate complete genome sequence analysis |
| 基金项目:公益性行业 (农业) 科研专项 (201303028); 安徽省农业科学院学科建设项目 (17A1125); 安徽省蔬菜产业技术体系 |
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| 中文摘要: |
| 利用酶联免疫和RT-PCR技术对采自安徽地区的蚕豆病株进行检测, 确定其病原为蚕豆萎蔫病毒2号 Broad bean wilt virus 2 (BBWV2) 。为明确BBWV2安徽分离物 (BBWV2-AH) 的分类地位, 克隆了该分离物的全基因组序列, 分析了其基因组特征。结果表明, BBWV2-AH RNA1全长为5 944 bp (GenBank登录号: KY606992), 含有1个ORF; BBWV2-AH RNA2全长3 587 bp (GenBank登录号: KY606993), 含有1个ORF。全序列核苷酸和氨基酸相似性分析显示, BBWV2-AH RNA1与BBWV2其他分离物的核苷酸、氨基酸相似性分别为78.4%~96%和87.1%~99%; BBWV2-AH RNA2与BBWV2其他分离物的核苷酸、氨基酸相似性分别为76.8%~95.5%和88.2%~98.3%。全基因组核苷酸序列系统发育分析显示, BBWV2-AH RNA1与中国的BBWV2-Hunan RNA1的亲缘关系最近, 而BBWV2-AH RNA2与韩国的多个分离物聚集在一起, 再与中国的分离物BBWV2-B935形成一个分支。 |
| 英文摘要: |
| BBWV2 was detected from broad bean plants in Anhui Province by DAS-ELISA and RT-PCR.To further characterize the BBWV2 Anhui isolate (BBWV2-AH), complete nucleotide sequence of BBWV2-AH was determined. Sequence analysis suggested that BBWV2-AH RNA1 was comprised of 5 944 nucleotides, encoding one ORF (GenBank accession no. KY606992); BBWV2-AH RNA2 was comprised of 3 587 nucleotides, also encoding one ORF (GenBank accession no. KY606993) . The comparison of nucleotide sequences suggested that RNA1 and RNA2 of BBWV2-AH shared 78.4%-96% and 76.8%-95.5% nucleotide sequence identity with those of other BBWV2 isolates, respectively. Furthermore, the comparison of amino acid sequences suggested that RNA1 and RNA2 of BBWV2-AH shared 87.1%-99% and 88.2%-98.3% amino acid sequence identity with those of other BBWV2 isolates, respectively. Phylogenetic analysis based on nucleotide sequences showed that RNA1 of BBWV2-AH isolate was most closely related to BBWV2-Hunan isolate, while RNA2 of BBWV2-AH formed an independent branch with several Korean isolates, and was then closely related to BBWV2-B935 from China. |
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